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Optics Letters

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  • Vol. 33, Iss. 9 — May. 1, 2008
  • pp: 1035–1037

Estimating the depth and lifetime of a fluorescent inclusion in a turbid medium using a simple time-domain optical method

Sung-Ho Han and David J. Hall  »View Author Affiliations


Optics Letters, Vol. 33, Issue 9, pp. 1035-1037 (2008)
http://dx.doi.org/10.1364/OL.33.001035


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Abstract

A simple time-domain optical method for estimating the depth ( d ) and lifetime ( τ ) of fluorescent inclusions in a turbid medium is described. We demonstrate the method for depth and lifetime estimation of a fluorescent inclusion directly by fitting a monoexponential decay ( τ eff ) of the temporal position of the temporal point-spread function and the measurement of its maximum temporal position ( t max ) . Since both of these measurements are intensity independent, this method provides a robust and efficient approach. This method is validated with experimental data.

© 2008 Optical Society of America

OCIS Codes
(170.0170) Medical optics and biotechnology : Medical optics and biotechnology
(170.3660) Medical optics and biotechnology : Light propagation in tissues
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(170.7050) Medical optics and biotechnology : Turbid media
(300.6280) Spectroscopy : Spectroscopy, fluorescence and luminescence

ToC Category:
Medical Optics and Biotechnology

History
Original Manuscript: January 4, 2008
Revised Manuscript: March 11, 2008
Manuscript Accepted: March 14, 2008
Published: April 30, 2008

Virtual Issues
Vol. 3, Iss. 6 Virtual Journal for Biomedical Optics

Citation
Sung-Ho Han and David J. Hall, "Estimating the depth and lifetime of a fluorescent inclusion in a turbid medium using a simple time-domain optical method," Opt. Lett. 33, 1035-1037 (2008)
http://www.opticsinfobase.org/ol/abstract.cfm?URI=ol-33-9-1035

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