OSA's Digital Library

Optics Letters

Optics Letters


  • Editor: Alan E. Willner
  • Vol. 34, Iss. 10 — May. 15, 2009
  • pp: 1525–1527

Effect of hemoglobin extinction spectra on optical spectroscopic measurements of blood oxygen saturation

Arjen Amelink, Theo Christiaanse, and Henricus J. C. M. Sterenborg  »View Author Affiliations

Optics Letters, Vol. 34, Issue 10, pp. 1525-1527 (2009)

View Full Text Article

Enhanced HTML    Acrobat PDF (229 KB)

Browse Journals / Lookup Meetings

Browse by Journal and Year


Lookup Conference Papers

Close Browse Journals / Lookup Meetings

Article Tools



Fiber-optic reflectance measurements were performed on scattering phantoms containing red blood cells. The oxygen pressure in the phantom was decreased by nitrogen gas bubbling, while carefully controlling and monitoring the temperature ( 37 ° C ) and pH (7.4). The blood oxygen saturation ( S O 2 ) was extracted from the optical spectroscopic measurements by fitting the spectra over the wavelength range of 350 850     nm to a model that includes the effects of pigment packaging. The effect of using different oxy- and deoxyhemoglobin extinction spectra on the quality and accuracy of the fits is analyzed. The optically extracted S O 2 is compared with the standard oxygen dissociation curve (ODC). Depending on the choice of hemoglobin extinction spectra the absolute deviation between the ODC and the optically extracted S O 2 is less than 2.5% over the measured range of saturations (4%–99%).

© 2009 Optical Society of America

OCIS Codes
(170.1470) Medical optics and biotechnology : Blood or tissue constituent monitoring
(170.4580) Medical optics and biotechnology : Optical diagnostics for medicine
(170.6510) Medical optics and biotechnology : Spectroscopy, tissue diagnostics
(170.7050) Medical optics and biotechnology : Turbid media

ToC Category:
Medical Optics and Biotechnology

Original Manuscript: January 23, 2009
Revised Manuscript: March 24, 2009
Manuscript Accepted: April 3, 2009
Published: May 8, 2009

Virtual Issues
Vol. 4, Iss. 7 Virtual Journal for Biomedical Optics

Arjen Amelink, Theo Christiaanse, and Henricus J. C. M. Sterenborg, "Effect of hemoglobin extinction spectra on optical spectroscopic measurements of blood oxygen saturation," Opt. Lett. 34, 1525-1527 (2009)

Sort:  Author  |  Year  |  Journal  |  Reset  


  1. T. J. Farrell, M. S. Patterson, and B. C. Wilson, Med. Phys. 19, 879 (1992). [CrossRef] [PubMed]
  2. G. M. Palmer, and N. Ramanujam, Appl. Opt. 45, 1062 (2006). [CrossRef] [PubMed]
  3. R. Reif, O. A'Amar, and I. J. Bigio, Appl. Opt. 46, 7317 (2007). [CrossRef] [PubMed]
  4. S. A. Prahl, http://omlc.ogi.edu/spectra/hemoglobin, retrieved September 2008.
  5. O. W. van Assendelft, Spectrophotometry of Haemoglobin Derivatives (Royal Van Gorcum Ltd., 1970).
  6. S. Takatani, and M. D. Graham, IEEE Trans. Biomed. Eng. BME-26, 656 (1987). [CrossRef]
  7. S. Wray, M. Cope, D. T. Delpy, J. S. Wyatt, and E. O. R. Reynolds, Biochem. Biophys. Acta. 933, 184 (1988). [CrossRef] [PubMed]
  8. W. G. Zijlstra, A. Buursma, and O. W. van Assendelft, Visible and Near Infrared Absorption Spectra of Human and Animal Haemoglobin (VSP BV, 2000).
  9. A. Amelink, D. J. Robinson, and H. J. C. M. Sterenborg, J. Biomed. Opt. 13, 054044 (2008). [CrossRef] [PubMed]
  10. A. Amelink and H. J. C. M. Sterenborg, Appl. Opt. 43, 3048 (2004). [CrossRef] [PubMed]
  11. S. C. Kanick, H. J. C. M. Sterenborg, and A. Amelink, J. Biomed. Opt. 13, 064042 (2008). [CrossRef]
  12. A. Amelink, O. P. Kaspers, H. J. C. M. Sterenborg, J. E. van der Wal, J. L. Roodenburg, and M. J. H. Witjes, Oral Oncol. 44, 65 (2008). [CrossRef]
  13. H. J. van Staveren, C. J. M. Moes, S. A. Prahl, and M. J. C. van Gemert, Appl. Opt. 30, 4507 (1991). [CrossRef] [PubMed]
  14. A. Amelink, M. P. L. Bard, J. A. Burgers and H. J. C. M. Sterenborg, Opt. Lett. 29, 1087 (2004). [CrossRef] [PubMed]
  15. J. W. Severinghaus, J. Appl. Physiol.: Respir., Environ. Exercise Physiol. 46, 599 (1979).
  16. J. C. Finlay and T. H. Foster, Med. Phys. 31, 1949 (2004). [CrossRef] [PubMed]
  17. M. Friebel, A. Roggan, G. Muller, and M. Meinke, J. Biomed. Opt. 11, 034021 (2006). [CrossRef]

Cited By

Alert me when this paper is cited

OSA is able to provide readers links to articles that cite this paper by participating in CrossRef's Cited-By Linking service. CrossRef includes content from more than 3000 publishers and societies. In addition to listing OSA journal articles that cite this paper, citing articles from other participating publishers will also be listed.


Fig. 1 Fig. 2 Fig. 3

« Previous Article  |  Next Article »

OSA is a member of CrossRef.

CrossCheck Deposited