Quantitative phase-gradient imaging at high resolution with asymmetric illumination-based differential phase contrast
Optics Letters, Vol. 34, Issue 13, pp. 1924-1926 doi:10.1364/OL.34.001924
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- OCIS Codes:
- (100.5070) Image processing : Phase retrieval
- (110.0180) Imaging systems : Microscopy
- (110.4850) Imaging systems : Optical transfer functions
- (110.4980) Imaging systems : Partial coherence in imaging
Imaging Systems
Citation
Shalin B. Mehta and Colin J. R. Sheppard, "Quantitative phase-gradient imaging at high resolution with asymmetric illumination-based differential phase contrast," Opt. Lett. 34, 1924-1926 (2009)
http://www.opticsinfobase.org/ol/abstract.cfm?URI=ol-34-13-1924
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We describe a full-field phase-gradient imaging method: asymmetric illumination-based differential phase contrast (AIDPC). Imaging properties of AIDPC are evaluated using the phase-gradient transfer-function approach and elucidated with experimental images of an optical fiber and a histochemical preparation of a skeletal muscle section. In comparison with full-field differential interference contrast, AIDPC does not require phase shifting for quantitative imaging of phase gradient, provides artifact-free images of birefringent specimens, requires shorter camera exposure, and has larger depth of focus. It is amenable to transfer-function engineering, simultaneous fluorescence imaging, and automated live cell imaging.
© 2009 Optical Society of America
» View Full Text: Acrobat PDF (204 KB)
History
Original Manuscript: March 30, 2009
Manuscript Accepted: May 24, 2009
Revised Manuscript: May 22, 2009
Published: June 18, 2009
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Author Affiliations
National University of Singapore
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