Spectroscopic phase microscopy for quantifying hemoglobin concentrations in intact red blood cells
Optics Letters, Vol. 34, Issue 23, pp. 3668-3670 (2009)
http://dx.doi.org/10.1364/OL.34.003668
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Abstract
We report a practical method for label-free quantification of specific molecules using spectroscopic imaging of sample-induced phase shifts. Diffraction phase microscopy equipped with various wavelengths of light source is used to record wavelength-dependent phase images. We first perform dispersion measurements on pure solutions of single molecular species present in the cells, such as albumin and hemoglobin (Hb). With this prior calibration of molecular specific dispersion, we demonstrate the extraction of Hb concentration from individual human red blood cells. The end point of this study is noninvasive monitoring of physiological states of intact living cells.
© 2009 Optical Society of America
OCIS Codes
(120.0120) Instrumentation, measurement, and metrology : Instrumentation, measurement, and metrology
(170.0170) Medical optics and biotechnology : Medical optics and biotechnology
(180.0180) Microscopy : Microscopy
ToC Category:
Microscopy
History
Original Manuscript: August 25, 2009
Manuscript Accepted: October 15, 2009
Published: November 20, 2009
Virtual Issues
Vol. 5, Iss. 1 Virtual Journal for Biomedical Optics
Citation
YongKeun Park, Toyohiko Yamauchi, Wonshik Choi, Ramachandra Dasari, and Michael S. Feld, "Spectroscopic phase microscopy for quantifying hemoglobin concentrations in intact red blood cells," Opt. Lett. 34, 3668-3670 (2009)
http://www.opticsinfobase.org/ol/abstract.cfm?URI=ol-34-23-3668
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