Abstract

Fourier transform second-harmonic generation (SHG) microscopy has been applied to quantitatively compare the information content between SHG images obtained from the forward and backward direction for three tissue types: porcine tendon, sclera, and ear cartilage. Both signal types yield consistent information on the preferred orientation of collagen fibers. For all specimens, the Fourier transform of the forward and backward SHG images produces several overlapping peaks in the magnitude spectrum at various depths into the tissues, indicating that some information present in the forward SHG images can be extracted from the backward SHG images. This study highlights the potential of backward SHG microscopy for medical diagnostics.

© 2009 Optical Society of America

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History
Original Manuscript: September 16, 2009
Manuscript Accepted: October 26, 2009
Revised Manuscript: October 26, 2009
Published: December 2, 2009

References

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Author Affiliations

Monal R. Mehta, Kimani C. Toussaint, Jr.

Department of Mechanical Science and Engineering, University of Illinois Urbana-Champaign, 1206 W Green Street, Urbana, Illinois 61801, USA

Raghu Ambekar Ramachandra Rao, Scott Leithem

Laboratory for Photonics Research of Bio/nano Environments (PROBE), Department of Electrical and Computer Engineering, University of Illinois Urbana-Champaign, 1406 W Green Street, Urbana, Illinois 61801, USA

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