Expand this Topic clickable element to expand a topic
Skip to content
Optica Publishing Group

Common-path multimodal optical microscopy

Not Accessible

Your library or personal account may give you access

Abstract

We have developed a common-path multimodal optical microscopy system that is capable of using a single optical source and a single camera to image amplitude, phase, and fluorescence features of a biological specimen. This is achieved by varying either contrast enhancement filters at the Fourier plane and/or neutral density/fluorescence filters in front of the CCD camera. The feasibility of the technique is demonstrated by obtaining brightfield, fluorescence, phase-contrast, spatially filtered, brightfield+fluorescence, phase+fluorescence, and edge-enhanced+fluorescence images of the same Drosophila embryo without the need for image registration and fusion. This comprehensive microscope has the capability of providing both structural and functional information and may be used for applications such as studying live-cell dynamics and in high throughput microscopy and automated microscopy.

© 2009 Optical Society of America

Full Article  |  PDF Article
More Like This
Common-path phase-shifting lensless holographic microscopy

Vicente Micó and Javier García
Opt. Lett. 35(23) 3919-3921 (2010)

Tomographic diffractive microscopy of living cells based on a common-path configuration

Wei-Chen Hsu, Jing-Wei Su, Te-Yu Tseng, and Kung-Bin Sung
Opt. Lett. 39(7) 2210-2213 (2014)

Simultaneous multimodal imaging with integrated photoacoustic microscopy and optical coherence tomography

Shuliang Jiao, Zhixing Xie, Hao F. Zhang, and Carmen A. Puliafito
Opt. Lett. 34(19) 2961-2963 (2009)

Cited By

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Figures (1)

You do not have subscription access to this journal. Figure files are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Select as filters


Select Topics Cancel
© Copyright 2024 | Optica Publishing Group. All Rights Reserved