OSA's Digital Library

Optics Letters

Optics Letters

| RAPID, SHORT PUBLICATIONS ON THE LATEST IN OPTICAL DISCOVERIES

  • Editor: Alan E. Willner
  • Vol. 35, Iss. 19 — Oct. 1, 2010
  • pp: 3195–3197

Subwavelength-resolution label-free photoacoustic microscopy of optical absorption in vivo

Chi Zhang, Konstantin Maslov, and Lihong V. Wang  »View Author Affiliations


Optics Letters, Vol. 35, Issue 19, pp. 3195-3197 (2010)
http://dx.doi.org/10.1364/OL.35.003195


View Full Text Article

Enhanced HTML    Acrobat PDF (508 KB)





Browse Journals / Lookup Meetings

Browse by Journal and Year


   


Lookup Conference Papers

Close Browse Journals / Lookup Meetings

Article Tools

Share
Citations

Abstract

Optical absorption provides essential biological functional information but cannot be sensed by mainstream optical microscopy technologies directly, which detect fluorescence or scattering and may require undesirable labeling. Here we developed in vivo subwavelength-resolution photoacoustic microscopy (SW-PAM) that provides exquisitely high optical-absorption contrast due to nonfluorescent, or fluorescent, endogenous pigments. Having approached the ultimate diffraction-limited optical resolution, SW-PAM can resolve subcellular organelles. Vasculature and early-stage melanoma were imaged with 12 : 1 and 17 : 1 contrasts, respectively, without labeling. SW-PAM along with the scaled-up macroscopy, as the only technology that measures the same contrast origin over such a wide length scale, can potentially accelerate translation from microscopic research to clinical practice.

© 2010 Optical Society of America

OCIS Codes
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(170.5120) Medical optics and biotechnology : Photoacoustic imaging
(180.5810) Microscopy : Scanning microscopy

ToC Category:
Medical Optics and Biotechnology

History
Original Manuscript: July 26, 2010
Revised Manuscript: August 27, 2010
Manuscript Accepted: August 31, 2010
Published: September 21, 2010

Virtual Issues
Vol. 5, Iss. 14 Virtual Journal for Biomedical Optics

Citation
Chi Zhang, Konstantin Maslov, and Lihong V. Wang, "Subwavelength-resolution label-free photoacoustic microscopy of optical absorption in vivo," Opt. Lett. 35, 3195-3197 (2010)
http://www.opticsinfobase.org/ol/abstract.cfm?URI=ol-35-19-3195


Sort:  Author  |  Year  |  Journal  |  Reset  

References

  1. L. V. Wang, Nat. Photonics 3, 503 (2009). [CrossRef]
  2. C. Li and L. V. Wang, Phys. Med. Biol. 54, R59 (2009). [CrossRef] [PubMed]
  3. H. F. Zhang, K. Maslov, G. Stoica, and L. V. Wang, Nat. Biotech. 24, 848 (2006). [CrossRef]
  4. K. Maslov, H. F. Zhang, S. Hu, and L. V. Wang, Opt. Lett. 33, 929 (2008). [CrossRef] [PubMed]
  5. J. Laufer, E. Zhang, G. Raivich, and P. Beard, Appl. Opt. 48, D299 (2009). [CrossRef] [PubMed]
  6. C. Kim, C. Favazza, and L. V. Wang, Chem. Rev. 110, 2756 (2010). [CrossRef] [PubMed]
  7. L. V. Wang and H. Wu, Biomedical Optics: Principles and Imaging (Wiley, 2007).
  8. L. V. Wang, IEEE J. Sel. Top. Quantum Electron. 14, 171 (2008). [CrossRef]
  9. V. Gray-Schopher, C. Wellbrock, and R. Marais, Nature 445, 851 (2007). [CrossRef]
  10. D. M. McDonald and P. L. Choyke, Nat. Med. 9, 713 (2003). [CrossRef] [PubMed]
  11. K. Kietzmann, D. Lubach, and H.-J. Heeren, Lab. Animal 24, 321 (1990). [CrossRef]
  12. W. Verkruysse, L. O. Svaasand, and W. Franco, J. Biomed. Opt. 14, 014005 (2009). [CrossRef] [PubMed]
  13. X. Wang, Y. Pang, G. Ku, X. Xie, G. Stoica, and L. V. Wang, Nat. Biotech. 21, 803 (2003). [CrossRef]
  14. M. P. Fronheiser, S. A. Ermilov, H. Brecht, A. Conjusteau, R. Su, K. Mehta, and A. A. Oraevsky, J. Biomed. Opt. 15, 021305 (2010). [CrossRef] [PubMed]
  15. L. Li, R. J. Zemp, G. Lungu, G. Stoica, and L. V. Wang, J. Biomed. Opt. 12, 020504 (2007). [CrossRef] [PubMed]
  16. K. H. Song, E. W. Stein, J. A. Margenthaler, and L. V. Wang, J. Biomed. Opt. 13, 054033 (2008). [CrossRef] [PubMed]

Cited By

Alert me when this paper is cited

OSA is able to provide readers links to articles that cite this paper by participating in CrossRef's Cited-By Linking service. CrossRef includes content from more than 3000 publishers and societies. In addition to listing OSA journal articles that cite this paper, citing articles from other participating publishers will also be listed.

Figures

Fig. 1 Fig. 2 Fig. 3
 
Fig. 4
 

« Previous Article  |  Next Article »

OSA is a member of CrossRef.

CrossCheck Deposited