We use a unique multifocal multiphoton microscope to directly characterize the pulse in the focal plane of a high-NA objective using second-harmonic generation frequency-resolved optical gating (FROG). Because of the nature of the optical setup, femtosecond laser pulses of orthogonal polarization states are generated in the focal plane, each acquiring a different spectral dispersion. By applying an additional constraint on the phase extraction algorithm, we simultaneously extract both the gate and probe pulses from a single spectrogram with a FROG error of 0.016.
© 2010 Optical Society of America
Original Manuscript: May 27, 2010
Revised Manuscript: August 3, 2010
Manuscript Accepted: August 19, 2010
Published: October 8, 2010
Jeffrey J. Field, Charles G. Durfee, and Jeff A. Squier, "Blind frequency-resolved optical-gating pulse characterization for quantitative differential multiphoton microscopy," Opt. Lett. 35, 3369-3371 (2010)