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Optics Letters

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  • Editor: Alan E. Willner
  • Vol. 35, Iss. 24 — Dec. 15, 2010
  • pp: 4096–4098

Multifocus confocal Raman microspectroscopy for fast multimode vibrational imaging of living cells

Masanari Okuno and Hiro-o Hamaguchi  »View Author Affiliations


Optics Letters, Vol. 35, Issue 24, pp. 4096-4098 (2010)
http://dx.doi.org/10.1364/OL.35.004096


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Abstract

We have developed a multifocus confocal Raman microspectroscopic system for the fast multimode vibrational imaging of living cells. It consists of an inverted microscope equipped with a microlens array, a pinhole array, a fiber bundle, and a multichannel Raman spectrometer. Forty-eight Raman spectra from 48 foci under the microscope are simultaneously obtained by using multifocus excitation and image-compression techniques. The multifocus confocal configuration suppresses the background generated from the cover glass and the cell culturing medium so that high-contrast images are obtainable with a short accumulation time. The system enables us to obtain multimode (10 different vibrational modes) vibrational images of living cells in tens of seconds with only 1 mW laser power at one focal point. This image acquisition time is more than 10 times faster than that in conventional single-focus Raman microspectroscopy.

© 2010 Optical Society of America

OCIS Codes
(110.0180) Imaging systems : Microscopy
(120.6200) Instrumentation, measurement, and metrology : Spectrometers and spectroscopic instrumentation
(290.5860) Scattering : Scattering, Raman
(300.6450) Spectroscopy : Spectroscopy, Raman

ToC Category:
Spectroscopy

History
Original Manuscript: August 26, 2010
Revised Manuscript: October 12, 2010
Manuscript Accepted: October 20, 2010
Published: December 2, 2010

Citation
Masanari Okuno and Hiro-o Hamaguchi, "Multifocus confocal Raman microspectroscopy for fast multimode vibrational imaging of living cells," Opt. Lett. 35, 4096-4098 (2010)
http://www.opticsinfobase.org/ol/abstract.cfm?URI=ol-35-24-4096


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