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Optics Letters

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  • Editor: Alan E. Willner
  • Vol. 35, Iss. 24 — Dec. 15, 2010
  • pp: 4102–4104

Label-free second-harmonic phase imaging of biological specimen by digital holographic microscopy

Etienne Shaffer, Corinne Moratal, Pierre Magistretti, Pierre Marquet, and Christian Depeursinge  »View Author Affiliations


Optics Letters, Vol. 35, Issue 24, pp. 4102-4104 (2010)
http://dx.doi.org/10.1364/OL.35.004102


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Abstract

We have previously developed a new way for nonscanning second-harmonic generation (SHG) microscopy [ Opt. Lett. 34, 2450 (2009)]. Based on digital holography, this technique captures, in single-shot hologram acquisition, both the amplitude and the phase of a coherent SHG radiation, which makes possible second harmonic phase microscopy. In this work, we present holographic SHG phase microscopy of a label-free biological tissue and discuss its added value to SHG microscopy.

© 2010 Optical Society of America

OCIS Codes
(110.0180) Imaging systems : Microscopy
(090.1995) Holography : Digital holography
(180.4315) Microscopy : Nonlinear microscopy

ToC Category:
Holography

History
Original Manuscript: August 27, 2010
Revised Manuscript: November 5, 2010
Manuscript Accepted: November 8, 2010
Published: December 3, 2010

Citation
Etienne Shaffer, Corinne Moratal, Pierre Magistretti, Pierre Marquet, and Christian Depeursinge, "Label-free second-harmonic phase imaging of biological specimen by digital holographic microscopy," Opt. Lett. 35, 4102-4104 (2010)
http://www.opticsinfobase.org/ol/abstract.cfm?URI=ol-35-24-4102


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