This Letter describes a quantitative phase microscopy for microfluidic devices using a simple self-referencing interferometry. Compared with the gross dimensions of the microfluidic device, the microchannel occupies only a small area of the device. Hence, the reference field can be generated by inverting the relative position of the specimen and background. Our system is realized using an extended depth-of-field optics in the form of Michelson interferometry, which allows quantitative phase measurement for an increased depth-of-field without moving objective lens or specimen. Furthermore, the system can be readily converted to a higher signal-to-noise ratio Hilbert phase microscopy thanks to the simultaneous acquisition of double interferograms. The performance of our system is verified using polymer beads, micropatterning poly(dimethylsiloxane) (PDMS), and embryo cells in the microchannels.
© 2010 Optical Society of America
Original Manuscript: October 23, 2009
Revised Manuscript: December 17, 2009
Manuscript Accepted: January 11, 2010
Published: February 9, 2010
Vol. 5, Iss. 5 Virtual Journal for Biomedical Optics
Jaeduck Jang, Chae Yun Bae, Je-Kyun Park, and Jong Chul Ye, "Self-reference quantitative phase microscopy for microfluidic devices," Opt. Lett. 35, 514-516 (2010)