Imaging of molecular probe activity with Born-normalized fluorescence optical projection tomography
Optics Letters, Vol. 35, Issue 7, pp. 1088-1090 (2010)
http://dx.doi.org/10.1364/OL.35.001088
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Abstract
Optical projection tomography is a new ex vivo imaging technique that allows imaging of whole organs in three dimensions at high spatial resolutions. In this Letter we demonstrate its capability to tomographically visualize molecular activity in whole organs of mice. In particular, eosinophil activity in asthmatic lungs is resolved using a Born-normalized fluorescence optical projection tomography and employing a near-IR molecular probe. The possibility to achieve molecularly sensitive imaging contrast in optical projection tomography by means of targeted and activatable imaging reporter agents adds a new range of capabilities for investigating molecular signatures of pathophysiological processes and a wide variety of diseases and their development.
© 2010 Optical Society of America
OCIS Codes
(110.0110) Imaging systems : Imaging systems
(170.0170) Medical optics and biotechnology : Medical optics and biotechnology
(170.3880) Medical optics and biotechnology : Medical and biological imaging
ToC Category:
Imaging Systems
History
Original Manuscript: December 22, 2009
Revised Manuscript: February 5, 2010
Manuscript Accepted: February 5, 2010
Published: March 31, 2010
Virtual Issues
Vol. 5, Iss. 8 Virtual Journal for Biomedical Optics
Citation
Claudio Vinegoni, Paolo Fumene Feruglio, Virna Cortez-Retamozo, Daniel Razansky, Benjamin D. Medoff, Vasilis Ntziachristos, Andrea Sbarbati, Mikael Pittet, and Ralph Weissleder, "Imaging of molecular probe activity with Born-normalized fluorescence optical projection tomography," Opt. Lett. 35, 1088-1090 (2010)
http://www.opticsinfobase.org/ol/abstract.cfm?URI=ol-35-7-1088
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