Scattered light fluorescence microscopy: imaging through turbid layers
Optics Letters, Vol. 35, Issue 8, pp. 1245-1247 (2010)
http://dx.doi.org/10.1364/OL.35.001245
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Abstract
A major limitation of any type of microscope is the penetration depth in turbid tissue. Here, we demonstrate a fundamentally novel kind of fluorescence microscope that images through optically thick turbid layers. The microscope uses scattered light, rather than light propagating along a straight path, for imaging with subwavelength resolution. Our method uses constructive interference to focus scattered laser light through the turbid layer. Microscopic fluorescent structures behind the layer were imaged by raster scanning the focus.
© 2010 Optical Society of America
OCIS Codes
(030.6140) Coherence and statistical optics : Speckle
(110.0180) Imaging systems : Microscopy
(110.7050) Imaging systems : Turbid media
(290.4210) Scattering : Multiple scattering
ToC Category:
Imaging Systems
History
Original Manuscript: January 4, 2010
Revised Manuscript: March 11, 2010
Manuscript Accepted: March 12, 2010
Published: April 15, 2010
Virtual Issues
Vol. 5, Iss. 9 Virtual Journal for Biomedical Optics
Citation
Ivo M. Vellekoop and Christof M. Aegerter, "Scattered light fluorescence microscopy: imaging through turbid layers," Opt. Lett. 35, 1245-1247 (2010)
http://www.opticsinfobase.org/ol/abstract.cfm?URI=ol-35-8-1245
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