OSA's Digital Library

Optics Letters

Optics Letters


  • Editor: Alan E. Willner
  • Vol. 35, Iss. 9 — May. 1, 2010
  • pp: 1374–1376

Investigation of portable in situ fluorescence optical detection for microfluidic 3D cell culture assays

Jong-ryul Choi, Jong Hwan Sung, Michael Louis Shuler, and Donghyun Kim  »View Author Affiliations

Optics Letters, Vol. 35, Issue 9, pp. 1374-1376 (2010)

View Full Text Article

Enhanced HTML    Acrobat PDF (283 KB)

Browse Journals / Lookup Meetings

Browse by Journal and Year


Lookup Conference Papers

Close Browse Journals / Lookup Meetings

Article Tools



A portable fluorescence optical detection system was developed to demonstrate real-time in situ analysis of cells that are three-dimensionally cultured in an extracellular matrix under microfluidic environment. The system was designed to provide a large field of view in the lateral plane to average out cellular processes in an axial layer and simultaneously diffraction-limited axial resolution. In this proof-of-concept study, the detection system was applied to quantitative analyses of short-term measurements of cell staining and cell cytotoxicity and long-term monitoring of a cell-invasion assay. For assays, colon cancer cells were cultured in a Matrigel or alginate matrix. The measured data were largely consistent with predicted results and revealed quantitatively cell dynamics specific to 3D cell cultures. The detection system has a potential as a single package to investigate 3D cultures in a microfluidic system.

© 2010 Optical Society of America

OCIS Codes
(120.3890) Instrumentation, measurement, and metrology : Medical optics instrumentation
(170.1530) Medical optics and biotechnology : Cell analysis
(280.4788) Remote sensing and sensors : Optical sensing and sensors

ToC Category:
Instrumentation, Measurement, and Metrology

Original Manuscript: January 11, 2010
Revised Manuscript: March 9, 2010
Manuscript Accepted: March 15, 2010
Published: April 23, 2010

Virtual Issues
Vol. 5, Iss. 9 Virtual Journal for Biomedical Optics

Jong-ryul Choi, Jong Hwan Sung, Michael Louis Shuler, and Donghyun Kim, "Investigation of portable in situ fluorescence optical detection for microfluidic 3D cell culture assays," Opt. Lett. 35, 1374-1376 (2010)

Sort:  Author  |  Year  |  Journal  |  Reset  


  1. A. Abbott, Nature 424, 870 (2003). [CrossRef] [PubMed]
  2. F. Pampaloni, E. G. Reynaud, and E. H. K. Stelzer, Nat. Rev. Mol. Cell Biol. 8, 839 (2007). [CrossRef] [PubMed]
  3. G. Y. Lee, P. A. Kenny, E. H. Lee, and M. J. Bissell, Nat. Methods 4, 359 (2007). [CrossRef] [PubMed]
  4. A. P. Wong, R. Perez-Castillejos, J. C. Love, and G. M. Whitesides, Biomaterials 29, 1853 (2008). [CrossRef] [PubMed]
  5. J. H. Sung, J. Choi, D. Kim, and M. L. Shuler, Biotechnol. Bioeng. 104, 516 (2009). [CrossRef] [PubMed]
  6. D. Tatosian, M. L. Shuler, and D. Kim, Opt. Lett. 30, 1689 (2005). [CrossRef] [PubMed]
  7. T. Oh, J. H. Sung, D. A. Tatosian, M. L. Shuler, and D. Kim, Cytometry, Part A 71A, 857 (2007). [CrossRef]
  8. N. B. E. Sawyer, L. K. Worrall, J. A. Crowe, S. L. Waters, K. M. Shakesheff, F. R. A. J. Rose, and S. P. Morgan, Biotechnol. Bioeng. 100, 159 (2008). [CrossRef]
  9. W. Tan, A. L. Oldenburg, J. J. Norman, T. A. Desai, and S. A. Boppart, Opt. Express 14, 7159 (2006). [CrossRef] [PubMed]
  10. S. Kwon and L. P. Lee, Opt. Lett. 29, 706 (2004). [CrossRef] [PubMed]
  11. S. Toetsch, P. Olwell, A. Prina-Mello, and Y. Volkov,Integr. Biol. 1, 170 (2009) [CrossRef]

Cited By

Alert me when this paper is cited

OSA is able to provide readers links to articles that cite this paper by participating in CrossRef's Cited-By Linking service. CrossRef includes content from more than 3000 publishers and societies. In addition to listing OSA journal articles that cite this paper, citing articles from other participating publishers will also be listed.


Fig. 1 Fig. 2 Fig. 3
Fig. 4

« Previous Article  |  Next Article »

OSA is a member of CrossRef.

CrossCheck Deposited