Phase retrieval is widely used in phase contrast microscopy. Here we present an autofocus algorithm that allows the phase of the exit wave function, from a single-material object, to be reconstructed at medium resolution from a single phase contrast image without any a priori knowledge of the imaging system or object. The algorithm is demonstrated on coherent out-of-focus electron micrographs of 30 nm latex sphere calibration standards, giving <10% RMS error over a large defocus range.
© 2011 Optical Society of America
Original Manuscript: February 15, 2011
Revised Manuscript: April 13, 2011
Manuscript Accepted: April 18, 2011
Published: May 13, 2011
Vol. 6, Iss. 6 Virtual Journal for Biomedical Optics
Samuel A. Eastwood, David M. Paganin, and Amelia C. Y. Liu, "Automated phase retrieval of a single-material object using a single out-of-focus image," Opt. Lett. 36, 1878-1880 (2011)