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Optics Letters

Optics Letters


  • Vol. 36, Iss. 10 — May. 15, 2011
  • pp: 1878–1880

Automated phase retrieval of a single-material object using a single out-of-focus image

Samuel A. Eastwood, David M. Paganin, and Amelia C. Y. Liu  »View Author Affiliations

Optics Letters, Vol. 36, Issue 10, pp. 1878-1880 (2011)

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Phase retrieval is widely used in phase contrast microscopy. Here we present an autofocus algorithm that allows the phase of the exit wave function, from a single-material object, to be reconstructed at medium resolution from a single phase contrast image without any a priori knowledge of the imaging system or object. The algorithm is demonstrated on coherent out-of-focus electron micrographs of 30 nm latex sphere calibration standards, giving < 10 % RMS error over a large defocus range.

© 2011 Optical Society of America

OCIS Codes
(030.1640) Coherence and statistical optics : Coherence
(100.3010) Image processing : Image reconstruction techniques
(100.5070) Image processing : Phase retrieval

ToC Category:
Image Processing

Original Manuscript: February 15, 2011
Revised Manuscript: April 13, 2011
Manuscript Accepted: April 18, 2011
Published: May 13, 2011

Virtual Issues
Vol. 6, Iss. 6 Virtual Journal for Biomedical Optics

Samuel A. Eastwood, David M. Paganin, and Amelia C. Y. Liu, "Automated phase retrieval of a single-material object using a single out-of-focus image," Opt. Lett. 36, 1878-1880 (2011)

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