OSA's Digital Library

Optics Letters

Optics Letters

| RAPID, SHORT PUBLICATIONS ON THE LATEST IN OPTICAL DISCOVERIES

  • Vol. 36, Iss. 15 — Aug. 1, 2011
  • pp: 2877–2879

Dielectric microsphere mediated transfection using a femtosecond laser

Mitsuhiro Terakawa and Yuto Tanaka  »View Author Affiliations


Optics Letters, Vol. 36, Issue 15, pp. 2877-2879 (2011)
http://dx.doi.org/10.1364/OL.36.002877


View Full Text Article

Enhanced HTML    Acrobat PDF (401 KB)





Browse Journals / Lookup Meetings

Browse by Journal and Year


   


Lookup Conference Papers

Close Browse Journals / Lookup Meetings

Article Tools

Share
Citations

Abstract

We demonstrate the permeabilization of cell membranes by an enhanced optical field generated under polystyrene microspheres of 1000 nm diameter excited by a femtosecond laser pulse. Fluorescent molecules and short interfering RNA (siRNA) have been successfully delivered to many cells in the irradiated area by a single 80 fs laser pulse at 800 nm wavelength in the presence of antibody-conjugated polystyrene spheres. The ratios of the cells showing permeabilization were 38% and 21% for Fluorescein isothiocyanate-dextran and siRNA, respectively, at the laser fluence of 1.06 J / cm 2 . The present method has advantages both in high throughput of many cell treatments and precise processing of minute areas on cell membranes.

© 2011 Optical Society of America

OCIS Codes
(140.3390) Lasers and laser optics : Laser materials processing
(170.0170) Medical optics and biotechnology : Medical optics and biotechnology
(290.4020) Scattering : Mie theory

ToC Category:
Lasers and Laser Optics

History
Original Manuscript: May 12, 2011
Manuscript Accepted: June 27, 2011
Published: July 25, 2011

Virtual Issues
Vol. 6, Iss. 9 Virtual Journal for Biomedical Optics

Citation
Mitsuhiro Terakawa and Yuto Tanaka, "Dielectric microsphere mediated transfection using a femtosecond laser," Opt. Lett. 36, 2877-2879 (2011)
http://www.opticsinfobase.org/ol/abstract.cfm?URI=ol-36-15-2877

You do not have subscription access to this journal. Citation lists with outbound citation links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Log in to access OSA Member Subscription

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Log in to access OSA Member Subscription

You do not have subscription access to this journal. Figure files are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Log in to access OSA Member Subscription

You do not have subscription access to this journal. Article level metrics are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Log in to access OSA Member Subscription

« Previous Article  |  Next Article »

OSA is a member of CrossRef.

CrossCheck Deposited