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Optics Letters

Optics Letters


  • Vol. 36, Iss. 15 — Aug. 1, 2011
  • pp: 2877–2879

Dielectric microsphere mediated transfection using a femtosecond laser

Mitsuhiro Terakawa and Yuto Tanaka  »View Author Affiliations

Optics Letters, Vol. 36, Issue 15, pp. 2877-2879 (2011)

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We demonstrate the permeabilization of cell membranes by an enhanced optical field generated under polystyrene microspheres of 1000 nm diameter excited by a femtosecond laser pulse. Fluorescent molecules and short interfering RNA (siRNA) have been successfully delivered to many cells in the irradiated area by a single 80 fs laser pulse at 800 nm wavelength in the presence of antibody-conjugated polystyrene spheres. The ratios of the cells showing permeabilization were 38% and 21% for Fluorescein isothiocyanate-dextran and siRNA, respectively, at the laser fluence of 1.06 J / cm 2 . The present method has advantages both in high throughput of many cell treatments and precise processing of minute areas on cell membranes.

© 2011 Optical Society of America

OCIS Codes
(140.3390) Lasers and laser optics : Laser materials processing
(170.0170) Medical optics and biotechnology : Medical optics and biotechnology
(290.4020) Scattering : Mie theory

ToC Category:
Lasers and Laser Optics

Original Manuscript: May 12, 2011
Manuscript Accepted: June 27, 2011
Published: July 25, 2011

Virtual Issues
Vol. 6, Iss. 9 Virtual Journal for Biomedical Optics

Mitsuhiro Terakawa and Yuto Tanaka, "Dielectric microsphere mediated transfection using a femtosecond laser," Opt. Lett. 36, 2877-2879 (2011)

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  1. M. Tsukakoshi, S. Kurata, Y. Nomiya, Y. Ikawa, and T. Kasuya, Appl. Phys. B 35, 135 (1984). [CrossRef]
  2. Y. Arita, M. L. Torres-Mapa, W. M. Lee, T. Čižmár, P. Campbell, F. J. Gunn-Moore, and K. Dholakia, Appl. Phys. Lett. 98, 093702 (2011). [CrossRef]
  3. M. L. Torres-Mapa, L. Angus, M. Ploschner, K. Dholakia, and F. J. Gunn-Moore, J. Biomed. Opt. 15, 041506 (2010). [CrossRef] [PubMed]
  4. U. K. Tirlapur and K. König, Nature 418, 290 (2002). [CrossRef] [PubMed]
  5. A. Vogel, J. Noack, G. Hüttman, and G. Paltauf, Appl. Phys. B 81, 1015 (2005). [CrossRef]
  6. S. Kumar, I. Z. Maxwell, A. Heisterkamp, T. R. Polte, T. P. Lele, M. Salanga, E. Mazur, and D. E. Ingber, Biophys. J. 90, 3762 (2006). [CrossRef] [PubMed]
  7. P. Mthunzi, K. Dholakia, and F. Gunn-Moore, J. Biomed. Opt. 15, 041507 (2010). [CrossRef] [PubMed]
  8. A. G. Doukas and N. Kollias, Adv. Drug Delivery Rev. 56, 559 (2004). [CrossRef]
  9. M. Terakawa, M. Ogura, S. Sato, H. Wakisaka, H. Ashida, M. Uenoyama, Y. Masaki, and M. Obara, Opt. Lett. 29, 1227 (2004). [CrossRef] [PubMed]
  10. W. R. Lee, S. C. Shen, C. R. Liu, C. L. Fang, C. H. Hu, and J. Y. Fang, J. Control. Release 115, 344 (2006). [CrossRef] [PubMed]
  11. T. H. Wu, S. Kalim, C. Callahan, M. Teitell, and P. Y. Chiou, Opt. Express 18, 938 (2010). [CrossRef] [PubMed]
  12. X. Huang, P. K. Jain, I. H. El-Sayed, and M. A. El-Sayed, Lasers Med. Sci. 23, 217 (2008). [CrossRef]
  13. C. M. Pitsillides, E. K. Joe, X. Wei, R. R. Anderson, and C. P. Lin, Biophys. J. 84, 4023 (2003). [CrossRef] [PubMed]
  14. C. Yao, X. Qu, Z. Zhang, G. Hüttman, and R. Rahmanzadeh, J. Biomed. Opt. 14, 054034 (2009). [CrossRef] [PubMed]
  15. J. Baumgart, L. Humbert, B. S. Lalonde, J. J. Lebrun, and M. Meunier, Proc. SPIE 7925, 79250I (2011). [CrossRef]
  16. M. Schomaker, J. Baumgart, D. Motekaitis, D. Heinemann, J. Kwawinkel, M. Pangalos, W. Bintig, E. Boulais, R. Lachaine, B. S. Lalonde, A. Ngezahayo, M. Meunier, and A. Heisterkamp, Proc. SPIE 7925, 79250F (2011). [CrossRef]
  17. Y. Pan, S. Neuss, A. Leifert, M. Fischler, F. Wen, U. Simon, G. Schmid, W. Brandau, and W. Jahnen-Dechent, Small 3, 1941 (2007). [CrossRef] [PubMed]
  18. Y. Chen, Y. Hung, I. Liau, and G. S. Huang, Nanoscale Res. Lett. 4, 858 (2009). [CrossRef] [PubMed]
  19. Z. B. Wang, J. Joseph, L. Li, and B. S. Luk’yanchuk, Proc. Inst. Mech. Eng. C 224, 1113 (2010). [CrossRef]
  20. T. Sakai, Y. Tanaka, Y. Nishizawa, M. Terakawa, and M. Obara, Appl. Phys. A 99, 39 (2010). [CrossRef]
  21. Y. Tanaka, G. Obara, A. Zenidaka, M. Terakawa, and M. Obara, Appl. Phys. Lett. 96, 201103 (2010). [CrossRef]
  22. G. Olivié, D. Giguère, F. Vidal, T. Ozaki, J.-C. Kieffer, O. Nada, and I. Brunette, Opt. Express 16, 4121 (2008). [CrossRef] [PubMed]
  23. M. F. Yanik, H. Cinar, H. N. Cinar, A. D. Chisholm, Y. Jin, and A. Ben-Yakar, Nature 432, 822 (2004). [CrossRef] [PubMed]
  24. A. Tien, S. Backus, H. Kapteyn, M. Murnane, and G. Mourou, Phys. Rev. Lett. 82, 3883 (1999). [CrossRef]

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