We demonstrate rapid vibrational imaging based on sum frequency generation (SFG) microscopy with a collinear excitation geometry. Using the tunable picosecond pulses from a high-repetition-rate optical parametric oscillator, vibrationally selective imaging of collagen fibers is achieved with submicrometer lateral resolution. We furthermore show simultaneous SFG and second harmonic generation imaging to emphasize the compatibility of the microscope with other nonlinear optical modalities.
© 2011 Optical Society of America
Original Manuscript: July 27, 2011
Revised Manuscript: August 22, 2011
Manuscript Accepted: September 6, 2011
Published: September 28, 2011
Vol. 6, Iss. 11 Virtual Journal for Biomedical Optics
Varun Raghunathan, Yang Han, Olaf Korth, Nien-Hui Ge, and Eric Olaf Potma, "Rapid vibrational imaging with sum frequency generation microscopy," Opt. Lett. 36, 3891-3893 (2011)