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  • Vol. 36, Iss. 6 — Mar. 15, 2011
  • pp: 834–836

Two-photon excited hemoglobin fluorescence provides contrast mechanism for label-free imaging of microvasculature in vivo

Dong Li, Wei Zheng, Yan Zeng, Yi Luo, and Jianan Y. Qu  »View Author Affiliations


Optics Letters, Vol. 36, Issue 6, pp. 834-836 (2011)
http://dx.doi.org/10.1364/OL.36.000834


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Abstract

Direct visualization of microvasculature provides significant insights in microcirculation and critically impacts the diagnosis and treatment of microcirculatory diseases. Recently, we discovered that the high-energy Soret fluorescence of hemoglobin peaked at 438 nm with an extremely short lifetime becomes strongly visible under two-photon excitation. Based on the distinct spectral and temporal characteristics of hemoglobin fluorescence, we demonstrated that two-photon fluorescence microscopy could become a powerful tool for label-free in vivo imaging of microvasculature in tissue.

© 2011 Optical Society of America

OCIS Codes
(170.1470) Medical optics and biotechnology : Blood or tissue constituent monitoring
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(170.6920) Medical optics and biotechnology : Time-resolved imaging
(180.6900) Microscopy : Three-dimensional microscopy
(180.4315) Microscopy : Nonlinear microscopy
(170.6935) Medical optics and biotechnology : Tissue characterization

ToC Category:
Medical Optics and Biotechnology

History
Original Manuscript: December 13, 2010
Revised Manuscript: January 16, 2011
Manuscript Accepted: February 10, 2011
Published: March 8, 2011

Virtual Issues
Vol. 6, Iss. 4 Virtual Journal for Biomedical Optics

Citation
Dong Li, Wei Zheng, Yan Zeng, Yi Luo, and Jianan Y. Qu, "Two-photon excited hemoglobin fluorescence provides contrast mechanism for label-free imaging of microvasculature in vivo," Opt. Lett. 36, 834-836 (2011)
http://www.opticsinfobase.org/ol/abstract.cfm?URI=ol-36-6-834


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References

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