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Optics Letters

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  • Vol. 36, Iss. 7 — Apr. 1, 2011
  • pp: 1029–1031

In vivo integrated photoacoustic and confocal microscopy of hemoglobin oxygen saturation and oxygen partial pressure

Yu Wang, Song Hu, Konstantin Maslov, Yu Zhang, Younan Xia, and Lihong V. Wang  »View Author Affiliations


Optics Letters, Vol. 36, Issue 7, pp. 1029-1031 (2011)
http://dx.doi.org/10.1364/OL.36.001029


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Abstract

We developed dual-modality microscope integrating photoacoustic microscopy (PAM) and fluorescence confocal microscopy (FCM) to noninvasively image hemoglobin oxygen saturation (sO2) and oxygen partial pressure (pO2) in vivo in single blood vessels with high spatial resolution. While PAM measures sO2 by imaging hemoglobin optical absorption at two wavelengths, FCM quantifies pO2 using phosphorescence quenching. The variations of sO2 and pO2 values in multiple orders of vessel branches under hyperoxic (100% oxygen) and normoxic (21% oxygen) conditions correlate well with the oxygen–hemoglobin dissociation curve. In addition, the total concentration of hemoglobin is imaged by PAM at an isosbestic wavelength.

© 2011 Optical Society of America

OCIS Codes
(170.1790) Medical optics and biotechnology : Confocal microscopy
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(170.5120) Medical optics and biotechnology : Photoacoustic imaging
(180.5810) Microscopy : Scanning microscopy

ToC Category:
Medical Optics and Biotechnology

History
Original Manuscript: December 15, 2010
Revised Manuscript: January 29, 2011
Manuscript Accepted: February 18, 2011
Published: March 16, 2011

Virtual Issues
Vol. 6, Iss. 5 Virtual Journal for Biomedical Optics

Citation
Yu Wang, Song Hu, Konstantin Maslov, Yu Zhang, Younan Xia, and Lihong V. Wang, "In vivo integrated photoacoustic and confocal microscopy of hemoglobin oxygen saturation and oxygen partial pressure," Opt. Lett. 36, 1029-1031 (2011)
http://www.opticsinfobase.org/ol/abstract.cfm?URI=ol-36-7-1029

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