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Optics Letters

Optics Letters

| RAPID, SHORT PUBLICATIONS ON THE LATEST IN OPTICAL DISCOVERIES

  • Vol. 36, Iss. 7 — Apr. 1, 2011
  • pp: 1062–1064

Adaptive optics confocal microscopy using direct wavefront sensing

Xiaodong Tao, Bautista Fernandez, Oscar Azucena, Min Fu, Denise Garcia, Yi Zuo, Diana C. Chen, and Joel Kubby  »View Author Affiliations


Optics Letters, Vol. 36, Issue 7, pp. 1062-1064 (2011)
http://dx.doi.org/10.1364/OL.36.001062


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Abstract

Optical aberrations due to the inhomogeneous refractive index of tissue degrade the resolution and brightness of images in deep-tissue imaging. We introduce a confocal fluorescence microscope with adaptive optics, which can correct aberrations based on direct wavefront measurements using a Shack–Hartmann wavefront sensor with a fluorescent bead used as a point source reference beacon. The results show a 4.3 × improvement in the Strehl ratio and a 240% improvement in the signal intensity for fixed mouse tissues at depths of up to 100 μm .

© 2011 Optical Society of America

OCIS Codes
(010.7350) Atmospheric and oceanic optics : Wave-front sensing
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(180.2520) Microscopy : Fluorescence microscopy
(180.6900) Microscopy : Three-dimensional microscopy
(110.1080) Imaging systems : Active or adaptive optics

ToC Category:
Microscopy

History
Original Manuscript: November 30, 2010
Revised Manuscript: February 6, 2011
Manuscript Accepted: February 22, 2011
Published: March 17, 2011

Virtual Issues
Vol. 6, Iss. 5 Virtual Journal for Biomedical Optics

Citation
Xiaodong Tao, Bautista Fernandez, Oscar Azucena, Min Fu, Denise Garcia, Yi Zuo, Diana C. Chen, and Joel Kubby, "Adaptive optics confocal microscopy using direct wavefront sensing," Opt. Lett. 36, 1062-1064 (2011)
http://www.opticsinfobase.org/ol/abstract.cfm?URI=ol-36-7-1062

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Supplementary Material


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