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Adaptive optics confocal microscopy using direct wavefront sensing |
Optics Letters, Vol. 36, Issue 7, pp. 1062-1064 (2011)
http://dx.doi.org/10.1364/OL.36.001062
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Abstract
Optical aberrations due to the inhomogeneous refractive index of tissue degrade the resolution and brightness of images in deep-tissue imaging. We introduce a confocal fluorescence microscope with adaptive optics, which can correct aberrations based on direct wavefront measurements using a Shack–Hartmann wavefront sensor with a fluorescent bead used as a point source reference beacon. The results show a
© 2011 Optical Society of America
OCIS Codes
(010.7350) Atmospheric and oceanic optics : Wave-front sensing
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(180.2520) Microscopy : Fluorescence microscopy
(180.6900) Microscopy : Three-dimensional microscopy
(110.1080) Imaging systems : Active or adaptive optics
ToC Category:
Microscopy
History
Original Manuscript: November 30, 2010
Revised Manuscript: February 6, 2011
Manuscript Accepted: February 22, 2011
Published: March 17, 2011
Virtual Issues
Vol. 6, Iss. 5 Virtual Journal for Biomedical Optics
Citation
Xiaodong Tao, Bautista Fernandez, Oscar Azucena, Min Fu, Denise Garcia, Yi Zuo, Diana C. Chen, and Joel Kubby, "Adaptive optics confocal microscopy using direct wavefront sensing," Opt. Lett. 36, 1062-1064 (2011)
http://www.opticsinfobase.org/ol/abstract.cfm?URI=ol-36-7-1062
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