We developed second-generation (G2) optical-resolution photoacoustic microscopy (OR-PAM). Incorporation of a novel acoustic detection scheme improved upon the sensitivity of our first-generation (G1) system by $18.4\mathrm{dB}$ , deepening the in vivo tissue penetration to $1.2\mathrm{mm}$ at $570\mathrm{nm}$ . Moreover, translating the imaging head instead of the living object accelerated the scanning speed by a factor of 5, widening the field of view within the same acquisition time. Mouse ears, as well as mouse brains with intact craniums, were imaged in vivo in both total concentration and oxygen saturation of hemoglobin.

© 2011 Optical Society of America

You do not have subscription access to this journal. Citation lists with outbound citation links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Log in to access OSA Member Subscription

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Log in to access OSA Member Subscription

You do not have subscription access to this journal. Figure files are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Log in to access OSA Member Subscription

You do not have subscription access to this journal. Article level metrics are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Log in to access OSA Member Subscription

Related Journal Articles

• In vivo dark-field reflection-mode photoacoustic microscopy (OL)
• Improved in vivo photoacoustic microscopy based on a virtual-detector concept (OL)
• In vivo volumetric imaging of subcutaneous microvasculature by photoacoustic microscopy (OE)
• Optical-resolution photoacoustic microscopy for in vivo imaging of single capillaries (OL)
• Photoacoustic endoscopy (OL)

Related Conference Papers

• Dynamic Visualization of Mitochondria in Cardiomyocytes with Third Harmonic Generation Microscopy
• Multi-photon microscopy of deep tissue with gradient index lenses
• Molecular Imaging of Central Nervous System with Multi-Modal Nonlinear Optical Microscopy
• Scanning thermoacoustic tomograhy in biological tissue
• Reflection-mode submicron-resolution photoacoustic microscopy in vivo