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Optics Letters

| RAPID, SHORT PUBLICATIONS ON THE LATEST IN OPTICAL DISCOVERIES

  • Vol. 37, Iss. 13 — Jul. 1, 2012
  • pp: 2481–2483

Fast and precise algorithm based on maximum radial symmetry for single molecule localization

Hongqiang Ma, Fan Long, Shaoqun Zeng, and Zhen-Li Huang  »View Author Affiliations


Optics Letters, Vol. 37, Issue 13, pp. 2481-2483 (2012)
http://dx.doi.org/10.1364/OL.37.002481


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Abstract

We present an algorithm to estimate the location of single fluorescent molecule with both high speed and high precision. This algorithm is based on finding the subpixel position with maximum radial symmetry in a pixelated single molecule fluorescence image. Compared with conventional algorithms, this algorithm does not rely on point-spread-function or noise model. Through numerical simulation and experimental analysis, we found that this algorithm exhibits localization precision very close to the maximum likelihood estimator (MLE), while executes 1000 times faster than the MLE and 6 times faster than the fluoroBancroft algorithm.

© 2012 Optical Society of America

OCIS Codes
(100.6640) Image processing : Superresolution
(110.2960) Imaging systems : Image analysis
(180.2520) Microscopy : Fluorescence microscopy

ToC Category:
Microscopy

History
Original Manuscript: March 2, 2012
Revised Manuscript: March 26, 2012
Manuscript Accepted: April 25, 2012
Published: June 19, 2012

Virtual Issues
Vol. 7, Iss. 9 Virtual Journal for Biomedical Optics

Citation
Hongqiang Ma, Fan Long, Shaoqun Zeng, and Zhen-Li Huang, "Fast and precise algorithm based on maximum radial symmetry for single molecule localization," Opt. Lett. 37, 2481-2483 (2012)
http://www.opticsinfobase.org/ol/abstract.cfm?URI=ol-37-13-2481

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