Second harmonic generation (SHG) is a well-established optical modality widely used in biomedical optics to image collagen based tissues. The coherent signal of the forward direction SHG produces a high resolution image that can resolve individual fibers (groups of fibrils). In highly ordered collagen lamellae, such as in the corneal stroma, it is important to determine the orientation of the fibers as they contribute significantly to the biomechanics of the tissue. However, due to the crimped structure of the fibers, it is challenging to robustly determine their orientation using an independent computational method, compared to the straight fibers problem. Previous work in the field used the polarization of the fundamental or other techniques involving a more manual selection of the orientation, in order to differentiate between various directions in corneal structures. Yet those lack accuracy and independency. We present a robust independent technique to determine the orientation of the fibers in the corneal structure. The experimental results presented here, taken from different lamellae, demonstrate strongly the correct orientation.

© 2012 Optical Society of America

You do not have subscription access to this journal. Citation lists with outbound citation links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Log in to access OSA Member Subscription

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Log in to access OSA Member Subscription

You do not have subscription access to this journal. Figure files are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Log in to access OSA Member Subscription

You do not have subscription access to this journal. Article level metrics are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Log in to access OSA Member Subscription

Related Journal Articles

• Phase-resolved optical coherence tomography and optical Doppler tomography for imaging blood flow in human skin with fast scanning speed and high velocity sensitivity (OL)
• Ultrasound tagged light imaging in turbid media in a reflectance geometry (OL)
• Edge-preserving sectional image reconstruction in optical scanning holography (JOSAA)
• Partially coherent lensfree tomographic microscopy [Invited] (AO)
• Super-resolution photon-efficient imaging by nanometric double-helix point spread function localization of emitters (SPINDLE) (OE)

Related Conference Papers

• PHOTOACOUSTIC IMAGING IN BIOMEDICINE
• PHOTOACOUSTIC IMAGING IN BIOMEDICINE
• Vibrational Photoacoustic Microscopy for Depth-resolved Bond-selective Imaging of Tissues and Organisms
• Vibrational Photoacoustic Microscopy for Depth-resolved Bond-selective Imaging of Tissues and Organisms
• PHOTOACOUSTIC IMAGING IN BIOMEDICINE
• PHOTOACOUSTIC IMAGING IN BIOMEDICINE
• Vibrational Photoacoustic Microscopy for Depth-resolved Bond-selective Imaging of Tissues and Organisms
• Vibrational Photoacoustic Microscopy for Depth-resolved Bond-selective Imaging of Tissues and Organisms
• PHOTOACOUSTIC IMAGING IN BIOMEDICINE
• PHOTOACOUSTIC IMAGING IN BIOMEDICINE