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Optics Letters

Optics Letters


  • Editor: Alan E. Willner
  • Vol. 37, Iss. 2 — Jan. 15, 2012
  • pp: 163–165

Toward single-molecule detection with sensors based on propagating surface plasmons

Pavel Kvasnička, Karel Chadt, Milan Vala, Markéta Bocková, and Jiří Homola  »View Author Affiliations

Optics Letters, Vol. 37, Issue 2, pp. 163-165 (2012)

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Surface plasmon resonance (SPR) sensors are known to be able to detect very low surface concentrations of (bio)molecules on macroscopic areas. To explore the potential of SPR biosensors to achieve single-molecule detection, we have minimized the read-out area (to 64μm2) by employing a sensor system based on spectroscopy of surface plasmons generated on a diffractive structure via a microscope objective and light collection through a small aperture. This approach allows for decreasing the number of detected molecules by 3 orders of magnitude compared to state-of-the-art SPR sensors. A protein monolayer has been shown to produce a response of 5000 times the baseline noise, suggesting that as few as 500 proteins could be detected by the sensor.

© 2012 Optical Society of America

OCIS Codes
(230.1950) Optical devices : Diffraction gratings
(240.0310) Optics at surfaces : Thin films
(240.6680) Optics at surfaces : Surface plasmons
(240.6690) Optics at surfaces : Surface waves
(240.6700) Optics at surfaces : Surfaces

ToC Category:
Optics at Surfaces

Original Manuscript: October 6, 2011
Revised Manuscript: November 20, 2011
Manuscript Accepted: November 21, 2011
Published: January 10, 2012

Virtual Issues
Vol. 7, Iss. 3 Virtual Journal for Biomedical Optics

Pavel Kvasnička, Karel Chadt, Milan Vala, Markéta Bocková, and Jiří Homola, "Toward single-molecule detection with sensors based on propagating surface plasmons," Opt. Lett. 37, 163-165 (2012)

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