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Optics Letters

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  • Editor: Alan E. Willner
  • Vol. 37, Iss. 3 — Feb. 1, 2012
  • pp: 440–442

Oriented imaging of 3D subcellular structures in bacterial cells using optical tweezers

G. Carmon, I. Fishov, and M. Feingold  »View Author Affiliations


Optics Letters, Vol. 37, Issue 3, pp. 440-442 (2012)
http://dx.doi.org/10.1364/OL.37.000440


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Abstract

Using oscillating optical tweezers, we show that controlled alignment of rod-shaped bacterial cells allows imaging fluorescently labeled three-dimensional (3D) subcellular structures from different, optimized viewpoints. To illustrate our method, we analyze the Z ring of E. coli. We obtain that the radial width of the Z ring in unconstricted cells is about 120 nm. This result suggests that the Z ring consists of an extremely sparse network of FtsZ filaments.

© 2012 Optical Society of America

OCIS Codes
(110.2960) Imaging systems : Image analysis
(180.6900) Microscopy : Three-dimensional microscopy

ToC Category:
Imaging Systems

History
Original Manuscript: September 15, 2011
Revised Manuscript: December 12, 2011
Manuscript Accepted: December 12, 2011
Published: February 1, 2012

Virtual Issues
Vol. 7, Iss. 4 Virtual Journal for Biomedical Optics

Citation
G. Carmon, I. Fishov, and M. Feingold, "Oriented imaging of 3D subcellular structures in bacterial cells using optical tweezers," Opt. Lett. 37, 440-442 (2012)
http://www.opticsinfobase.org/ol/abstract.cfm?URI=ol-37-3-440


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