Using oscillating optical tweezers, we show that controlled alignment of rod-shaped bacterial cells allows imaging fluorescently labeled three-dimensional (3D) subcellular structures from different, optimized viewpoints. To illustrate our method, we analyze the Z ring of E. coli. We obtain that the radial width of the Z ring in unconstricted cells is about 120 nm. This result suggests that the Z ring consists of an extremely sparse network of FtsZ filaments.
© 2012 Optical Society of America
Original Manuscript: September 15, 2011
Revised Manuscript: December 12, 2011
Manuscript Accepted: December 12, 2011
Published: February 1, 2012
Vol. 7, Iss. 4 Virtual Journal for Biomedical Optics
G. Carmon, I. Fishov, and M. Feingold, "Oriented imaging of 3D subcellular structures in bacterial cells using optical tweezers," Opt. Lett. 37, 440-442 (2012)