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Near-field penetrating optical microscopy: a live cell nanoscale refractive index measurement technique for quantification of internal macromolecular density |
Optics Letters, Vol. 37, Issue 4, pp. 506-508 (2012)
http://dx.doi.org/10.1364/OL.37.000506
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Abstract
Quantification of intracellular nanoscale macromolecular density distribution is a fundamental aspect to understanding cellular processes. We report a near-field penetrating optical microscopy (NPOM) technique to directly probe the internal nanoscale macromolecular density of biological cells through quantification of intracellular refractive index (RI). NPOM inserts a tapered optical fiber probe to successive depths into an illuminated sample. A
© 2012 Optical Society of America
OCIS Codes
(120.5710) Instrumentation, measurement, and metrology : Refraction
(170.0180) Medical optics and biotechnology : Microscopy
(170.1530) Medical optics and biotechnology : Cell analysis
(180.4243) Microscopy : Near-field microscopy
ToC Category:
Medical Optics and Biotechnology
History
Original Manuscript: October 6, 2011
Revised Manuscript: December 13, 2011
Manuscript Accepted: December 15, 2011
Published: February 8, 2012
Virtual Issues
Vol. 7, Iss. 4 Virtual Journal for Biomedical Optics
Citation
Samantha Dale Strasser, Gajendra Shekhawat, Jeremy D. Rogers, Vinayak P. Dravid, Allen Taflove, and Vadim Backman, "Near-field penetrating optical microscopy: a live cell nanoscale refractive index measurement technique for quantification of internal macromolecular density," Opt. Lett. 37, 506-508 (2012)
http://www.opticsinfobase.org/ol/abstract.cfm?URI=ol-37-4-506
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