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Label-free 3D imaging of microstructure, blood, and lymphatic vessels within tissue beds in vivo |
Optics Letters, Vol. 37, Issue 5, pp. 812-814 (2012)
http://dx.doi.org/10.1364/OL.37.000812
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Abstract
This Letter reports the use of an ultrahigh resolution optical microangiography (OMAG) system for simultaneous 3D imaging of microstructure and lymphatic and blood vessels without the use of an exogenous contrast agent. An automatic algorithm is developed to segment the lymphatic vessels from the microstructural images based on the fact that the lymph fluid is optically transparent. An OMAG system is developed that utilizes a broadband supercontinuum light source, providing an axial resolution of 2.3 μm and lateral resolution of 5.8 μm, capable of resolving the capillary vasculature and lymphatic vessels innervating microcirculatory tissue beds. Experimental demonstration is performed by showing detailed 3D lymphatic and blood vessel maps, coupled with morphology, within mouse ears in vivo.
© 2012 Optical Society of America
OCIS Codes
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(170.4500) Medical optics and biotechnology : Optical coherence tomography
ToC Category:
Medical Optics and Biotechnology
History
Original Manuscript: December 8, 2011
Revised Manuscript: January 14, 2012
Manuscript Accepted: January 16, 2012
Published: February 21, 2012
Virtual Issues
Vol. 7, Iss. 5 Virtual Journal for Biomedical Optics
Citation
Zhongwei Zhi, Yeongri Jung, and Ruikang K. Wang, "Label-free 3D imaging of microstructure, blood, and lymphatic vessels within tissue beds in vivo," Opt. Lett. 37, 812-814 (2012)
http://www.opticsinfobase.org/ol/abstract.cfm?URI=ol-37-5-812
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