OSA's Digital Library

Optics Letters

Optics Letters


  • Editor: Alan E. Willner
  • Vol. 38, Iss. 12 — Jun. 15, 2013
  • pp: 2038–2040

Resolution below the point spread function for diffuse optical imaging using fluorescence lifetime multiplexing

William L. Rice, Steven Hou, and Anand T. N. Kumar  »View Author Affiliations

Optics Letters, Vol. 38, Issue 12, pp. 2038-2040 (2013)

View Full Text Article

Enhanced HTML    Acrobat PDF (293 KB)

Browse Journals / Lookup Meetings

Browse by Journal and Year


Lookup Conference Papers

Close Browse Journals / Lookup Meetings

Article Tools



We show that asymptotic lifetime-based fluorescence tomography can localize multiple-lifetime targets separated well below the diffuse point spread function of a turbid medium. This is made possible due to a complete diagonalization of the time domain forward problem in the asymptotic limit. We also show that continuous wave or direct time gate approaches to fluorescence tomography are unable to achieve this separation, indicating the unique advantage of a decay-amplitude-based approach for tomographic lifetime multiplexing with time domain data.

© 2013 Optical Society of America

OCIS Codes
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(170.6920) Medical optics and biotechnology : Time-resolved imaging
(170.6960) Medical optics and biotechnology : Tomography

ToC Category:
Medical Optics and Biotechnology

Original Manuscript: April 12, 2013
Manuscript Accepted: May 8, 2013
Published: June 4, 2013

Virtual Issues
Vol. 8, Iss. 7 Virtual Journal for Biomedical Optics

William L. Rice, Steven Hou, and Anand T. N. Kumar, "Resolution below the point spread function for diffuse optical imaging using fluorescence lifetime multiplexing," Opt. Lett. 38, 2038-2040 (2013)

Sort:  Author  |  Year  |  Journal  |  Reset  


  1. E. E. Graves, J. Ripoll, R. Weissleder, and V. Ntziachristos, Med. Phys. 30, 901 (2003). [CrossRef]
  2. X. Gao, Y. Cui, R. M. Levenson, L. W. K. Chung, and S. Nie, Nat. Biotechnol. 22, 969 (2004). [CrossRef]
  3. A. Godavarty, E. M. Sevick-Muraca, and M. J. Eppstein, Med. Phys. 32, 992 (2005). [CrossRef]
  4. J. R. Lakowicz, Principles of Fluorescence Spectroscopy, 3rd ed. (Springer, 2006), pp. 157–199.
  5. S. B. Raymond, D. A. Boas, B. J. Bacskai, and A. T. N. Kumar, J. Biomed. Opt. 15, 046011 (2010). [CrossRef]
  6. R. E. Nothdurft, S. V. Patwardhan, W. Akers, Y. Ye, S. Achilefu, and J. P. Culver, J. Biomed. Opt. 14, 024004 (2009). [CrossRef]
  7. Z. Li and M. Niedre, Biomed. Opt. Express 2665 (2011). [CrossRef]
  8. F. Leblond, H. Dehghani, D. Kepshire, and B. W. Pogue, J. Opt. Soc. Am. A 26, 1444 (2009). [CrossRef]
  9. A. T. N. Kumar, E. Chung, S. B. Raymond, J. A. J. M. van de Water, K. Shah, D. Fukumura, R. K. Jain, B. J. Bacskai, and D. A. Boas, Opt. Lett. 34, 2066 (2009). [CrossRef]
  10. A. T. N. Kumar, S. B. Raymond, A. K. Dunn, B. J. Bacskai, and D. A. Boas, IEEE Trans. Med. Imaging 27, 1152 (2008). [CrossRef]
  11. A. T. N. Kumar, S. B. Raymond, B. J. Bacskai, and D. A. Boas, Opt. Lett. 33, 470 (2008). [CrossRef]
  12. A. T. N. Kumar, S. B. Raymond, G. Boverman, D. A. Boas, and B. J. Bacskai, Opt. Express 14, 12255 (2006). [CrossRef]
  13. B. W. Pogue, S. C. Davis, X. Song, B. A. Brooksby, H. Dehghani, and K. D. Paulsen, J. Biomed. Opt. 11, 33001 (2006). [CrossRef]
  14. J. Chen, V. Venugopal, and X. Intes, Biomed. Opt. Express 2, 871 (2011). [CrossRef]

Cited By

Alert me when this paper is cited

OSA is able to provide readers links to articles that cite this paper by participating in CrossRef's Cited-By Linking service. CrossRef includes content from more than 3000 publishers and societies. In addition to listing OSA journal articles that cite this paper, citing articles from other participating publishers will also be listed.


Fig. 1. Fig. 2. Fig. 3.

« Previous Article  |  Next Article »

OSA is a member of CrossRef.

CrossCheck Deposited