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Optics Letters

Optics Letters

| RAPID, SHORT PUBLICATIONS ON THE LATEST IN OPTICAL DISCOVERIES

  • Editor: Alan E. Willner
  • Vol. 38, Iss. 15 — Aug. 1, 2013
  • pp: 2889–2892

Superresolution by image scanning microscopy using pixel reassignment

Colin J. R. Sheppard, Shalin B. Mehta, and Rainer Heintzmann  »View Author Affiliations


Optics Letters, Vol. 38, Issue 15, pp. 2889-2892 (2013)
http://dx.doi.org/10.1364/OL.38.002889


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Abstract

The effect of detector array size on resolution and signal collection efficiency of image scanning microscopy based on pixel reassignment is studied. It is shown how the method can also be employed if there is a Stokes shift in fluorescence emission wavelength. With no Stokes shift, the width of the point spread function can be sharpened by a factor of 1.53, and its peak intensity increased by a factor of 1.84.

© 2013 Optical Society of America

OCIS Codes
(170.1790) Medical optics and biotechnology : Confocal microscopy
(180.2520) Microscopy : Fluorescence microscopy
(180.5810) Microscopy : Scanning microscopy

ToC Category:
Microscopy

History
Original Manuscript: June 12, 2013
Manuscript Accepted: June 26, 2013
Published: July 30, 2013

Virtual Issues
Vol. 8, Iss. 9 Virtual Journal for Biomedical Optics

Citation
Colin J. R. Sheppard, Shalin B. Mehta, and Rainer Heintzmann, "Superresolution by image scanning microscopy using pixel reassignment," Opt. Lett. 38, 2889-2892 (2013)
http://www.opticsinfobase.org/ol/abstract.cfm?URI=ol-38-15-2889

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Supplementary Material


» Media 1: AVI (3246 KB)     

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