The effect of detector array size on resolution and signal collection efficiency of image scanning microscopy based on pixel reassignment is studied. It is shown how the method can also be employed if there is a Stokes shift in fluorescence emission wavelength. With no Stokes shift, the width of the point spread function can be sharpened by a factor of 1.53, and its peak intensity increased by a factor of 1.84.
© 2013 Optical Society of America
Original Manuscript: June 12, 2013
Manuscript Accepted: June 26, 2013
Published: July 30, 2013
Vol. 8, Iss. 9 Virtual Journal for Biomedical Optics
Colin J. R. Sheppard, Shalin B. Mehta, and Rainer Heintzmann, "Superresolution by image scanning microscopy using pixel reassignment," Opt. Lett. 38, 2889-2892 (2013)
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