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Optics Letters

Optics Letters


  • Editor: Alan E. Willner
  • Vol. 38, Iss. 17 — Sep. 1, 2013
  • pp: 3402–3405

Laser-induced breakdown of an optically trapped gold nanoparticle for single cell transfection

Yoshihiko Arita, Martin Ploschner, Maciej Antkowiak, Frank Gunn-Moore, and Kishan Dholakia  »View Author Affiliations

Optics Letters, Vol. 38, Issue 17, pp. 3402-3405 (2013)

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The cell selective introduction of therapeutic agents remains a challenging problem. Here we demonstrate spatially controlled cavitation instigated by laser-induced breakdown of an optically trapped single gold nanoparticle of diameter 100 nm. The energy breakdown threshold of the gold nanoparticle with a single nanosecond laser pulse at 532 nm is three orders of magnitude lower than water, which leads to nanocavitation allowing single cell transfection. We quantify the shear stress to cells from the expanding bubble and optimize the pressure to be in the range of 1 10 kPa for transfection. The method shows transfection of plasmid DNA into individual mammalian cells with an efficiency of 75%.

© 2013 Optical Society of America

OCIS Codes
(140.3440) Lasers and laser optics : Laser-induced breakdown
(140.7010) Lasers and laser optics : Laser trapping
(170.7170) Medical optics and biotechnology : Ultrasound
(240.6680) Optics at surfaces : Surface plasmons

ToC Category:
Lasers and Laser Optics

Original Manuscript: June 10, 2013
Revised Manuscript: August 1, 2013
Manuscript Accepted: August 1, 2013
Published: August 28, 2013

Virtual Issues
Vol. 8, Iss. 10 Virtual Journal for Biomedical Optics

Yoshihiko Arita, Martin Ploschner, Maciej Antkowiak, Frank Gunn-Moore, and Kishan Dholakia, "Laser-induced breakdown of an optically trapped gold nanoparticle for single cell transfection," Opt. Lett. 38, 3402-3405 (2013)

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