|
Multicolor stimulated Raman scattering microscopy with a rapidly tunable optical parametric oscillator |
Optics Letters, Vol. 38, Issue 2, pp. 145-147 (2013)
http://dx.doi.org/10.1364/OL.38.000145
Enhanced HTML 
Acrobat PDF (260 KB)
Abstract
Stimulated Raman scattering (SRS) microscopy allows label-free chemical imaging based on vibrational spectroscopy. Narrowband excitation with picosecond lasers creates the highest signal levels and enables imaging speeds up to video-rate, but it sacrifices chemical specificity in samples with overlapping bands compared to broadband (multiplex) excitation. We develop a rapidly tunable picosecond optical parametric oscillator with an electro-optical tunable Lyot filter, and demonstrate multicolor SRS microscopy with synchronized line-by-line wavelength tuning to avoid spectral artifacts due to sample movement. We show sensitive imaging of three different kinds of polymer beads and live HeLa cells with moving intracellular lipid droplets.
© 2013 Optical Society of America
OCIS Codes
(190.4970) Nonlinear optics : Parametric oscillators and amplifiers
(180.4315) Microscopy : Nonlinear microscopy
(180.5655) Microscopy : Raman microscopy
ToC Category:
Microscopy
History
Original Manuscript: November 8, 2012
Manuscript Accepted: November 30, 2012
Published: January 8, 2013
Virtual Issues
Vol. 8, Iss. 2 Virtual Journal for Biomedical Optics
Citation
Lingjie Kong, Minbiao Ji, Gary R. Holtom, Dan Fu, Christian W. Freudiger, and X. Sunney Xie, "Multicolor stimulated Raman scattering microscopy with a rapidly tunable optical parametric oscillator," Opt. Lett. 38, 145-147 (2013)
http://www.opticsinfobase.org/ol/abstract.cfm?URI=ol-38-2-145
You do not have subscription access to this journal. Citation lists with outbound citation links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.
Contact your librarian or system administrator
or
Log in to access OSA Member Subscription
You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.
Contact your librarian or system administrator
or
Log in to access OSA Member Subscription
You do not have subscription access to this journal. Figure files are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.
Contact your librarian or system administrator
or
Log in to access OSA Member Subscription
You do not have subscription access to this journal. Article level metrics are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.
Contact your librarian or system administrator
or
Log in to access OSA Member Subscription
Related Journal Articles 
- Ultrabroadband multiplex CARS microspectroscopy and imaging using a subnanosecond supercontinuum light source in the deep near infrared (OL)
- Comparing coherent and spontaneous Raman scattering under biological imaging conditions (OL)
- A 75 MHz Light Source for Femtosecond Stimulated Raman Microscopy (OE)
- Coherent anti-Stokes Raman scattering microscopy using a single-pass picosecond supercontinuum-seeded optical parametric amplifier (OE)
- In vivo evaluation of demyelination and remyelination in a nerve crush injury model (BOE)
Related Conference Papers
- Coherent Anti-Stokes Raman Scattering (CARS) Microscopy for Three-Dimensional Flow Characterization in Microfluidics
- Comparing Coherent and Spontaneous Raman Scattering Under Biological Imaging Conditions
- Nonlinear Microspectroscopy for Biomedical Applications
- Development of Transient Absorption Ultrasonic Microscopy
- Development of Transient Absorption Ultrasonic Microscopy
- Fiber-format Picosecond Source for Coherent Raman Microscopy
« Previous Article | Next Article »
OSA is a member of 