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Optics Letters

Optics Letters


  • Editor: Alan E. Willner
  • Vol. 38, Iss. 22 — Nov. 15, 2013
  • pp: 4923–4926

Calibration of optical traps by dual trapping of one bead

Pavel Dutov and Jay Schieber  »View Author Affiliations

Optics Letters, Vol. 38, Issue 22, pp. 4923-4926 (2013)

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We introduce a method for optical trap calibration that is suitable for viscoelastic material. The method is designed for use on experimental setups with two optical tweezers and is based on pulling a trapped particle with one trap while holding it with the other. No piezo stage is needed, and only one optical trap must be movable with galvo mirrors, piezo mirrors, or acousto-optical deflectors. The method combines advantages of commonly known PSD-fitting and fast-sweeping methods, allowing calibration of a completely fixed trap in a fluid of unknown viscosity/viscoelasticity. A detailed method description, a theoretical derivation, and an experimental comparison to other methods are reported.

© 2013 Optical Society of America

OCIS Codes
(120.0120) Instrumentation, measurement, and metrology : Instrumentation, measurement, and metrology
(140.7010) Lasers and laser optics : Laser trapping

ToC Category:
Instrumentation, Measurement, and Metrology

Original Manuscript: August 21, 2013
Revised Manuscript: October 16, 2013
Manuscript Accepted: October 16, 2013
Published: November 15, 2013

Virtual Issues
Vol. 9, Iss. 1 Virtual Journal for Biomedical Optics

Pavel Dutov and Jay Schieber, "Calibration of optical traps by dual trapping of one bead," Opt. Lett. 38, 4923-4926 (2013)

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