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Optics Letters

Optics Letters


  • Editor: Alan E. Willner
  • Vol. 38, Iss. 23 — Dec. 1, 2013
  • pp: 5075–5078

Adaptive optical two-photon microscopy using autofluorescent guide stars

Xiaodong Tao, Andrew Norton, Matthew Kissel, Oscar Azucena, and Joel Kubby  »View Author Affiliations

Optics Letters, Vol. 38, Issue 23, pp. 5075-5078 (2013)

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We demonstrate a fast, direct wavefront-sensing method for dynamic in vivo adaptive optical two-photon microscopy. By using a Shack–Hartmann wavefront sensor and open-loop control, the system provides high-speed wavefront measurement and correction. To measure the wavefront in the middle of a Drosophila embryo at early stages, autofluorescence from endogenous fluorophores in the yolk were used as reference guide stars. The method was tested through live imaging of a Drosophila embryo. The aberration in the middle of the embryo was measured directly for the first time. After correction, the contrast and signal intensity of the structure in the middle of the embryo was improved.

© 2013 Optical Society of America

OCIS Codes
(010.7350) Atmospheric and oceanic optics : Wave-front sensing
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(180.2520) Microscopy : Fluorescence microscopy
(180.6900) Microscopy : Three-dimensional microscopy
(110.1080) Imaging systems : Active or adaptive optics

ToC Category:

Original Manuscript: July 29, 2013
Revised Manuscript: October 19, 2013
Manuscript Accepted: October 22, 2013
Published: November 25, 2013

Virtual Issues
Vol. 9, Iss. 2 Virtual Journal for Biomedical Optics

Xiaodong Tao, Andrew Norton, Matthew Kissel, Oscar Azucena, and Joel Kubby, "Adaptive optical two-photon microscopy using autofluorescent guide stars," Opt. Lett. 38, 5075-5078 (2013)

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