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Dispersion management in two-photon microscopy by using diffractive optical elements |
Optics Letters, Vol. 38, Issue 4, pp. 440-442 (2013)
http://dx.doi.org/10.1364/OL.38.000440
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Abstract
We demonstrate efficient generation of wide-field fluorescence signals in two-photon microscopy exploiting diffractive optical elements and short pulses by using a dispersion-compensated beam delivery optics module. Computer-generated holograms are codified onto a phase-only spatial light modulator, which allows for arbitrary single-shot patterning of the sample. Spatiotemporal shaping of the pulse is mandatory to overcome spatial chirp and pulse-front tilt effects that spread both in space and time the irradiance patterns, thus limiting not only the spatial resolution but also the signal-to-noise ratio in two-photon microscopy. By using a multipass amplifier delivering 30 fs, 0.8 mJ pulses at 1 kHz repetition rate, we experimentally demonstrated arbitrary single-shot fluorescence irradiance patterns in Rhodamine B.
© 2013 Optical Society of America
OCIS Codes
(090.1970) Holography : Diffractive optics
(190.4180) Nonlinear optics : Multiphoton processes
(180.4315) Microscopy : Nonlinear microscopy
ToC Category:
Ultrafast Optics
History
Original Manuscript: November 30, 2012
Revised Manuscript: January 3, 2013
Manuscript Accepted: January 6, 2013
Published: February 8, 2013
Virtual Issues
Vol. 8, Iss. 3 Virtual Journal for Biomedical Optics
Citation
Jorge Pérez-Vizcaíno, Omel Mendoza-Yero, Gladys Mínguez-Vega, Raúl Martínez-Cuenca, Pedro Andrés, and Jesús Lancis, "Dispersion management in two-photon microscopy by using diffractive optical elements," Opt. Lett. 38, 440-442 (2013)
http://www.opticsinfobase.org/ol/abstract.cfm?URI=ol-38-4-440
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