We proposed a subtraction method using vector beams for resolution enhancement in confocal microscopy. The imaging simulation revealed that the negative side lobe due to the excess subtraction resulted in the degradation of the object image. The subtraction imaging using vector beams demonstrated high spatial resolution with avoiding the negative side lobe. Further resolution enhancement beyond 100 nm was predicted by using a flat-top beam obtained by the combination of beams with radial and azimuthal polarizations and a higher-order transverse mode azimuthally polarized beam without significant negative side lobe.
© 2014 Optical Society of America
Original Manuscript: March 12, 2014
Revised Manuscript: March 31, 2014
Manuscript Accepted: April 14, 2014
Published: May 19, 2014
Vol. 9, Iss. 8 Virtual Journal for Biomedical Optics
Susumu Segawa, Yuichi Kozawa, and Shunichi Sato, "Resolution enhancement of confocal microscopy by subtraction method with vector beams," Opt. Lett. 39, 3118-3121 (2014)