OSA's Digital Library

Optics Letters

Optics Letters

| RAPID, SHORT PUBLICATIONS ON THE LATEST IN OPTICAL DISCOVERIES

  • Editor: Xi-Cheng Zhang
  • Vol. 39, Iss. 11 — Jun. 1, 2014
  • pp: 3134–3137

Temporal focusing-based multiphoton excitation microscopy via digital micromirror device

Jenq-Nan Yih, Yvonne Yuling Hu, Yong Da Sie, Li-Chung Cheng, Chi-Hsiang Lien, and Shean-Jen Chen  »View Author Affiliations


Optics Letters, Vol. 39, Issue 11, pp. 3134-3137 (2014)
http://dx.doi.org/10.1364/OL.39.003134


View Full Text Article

Enhanced HTML    Acrobat PDF (366 KB)





Browse Journals / Lookup Meetings

Browse by Journal and Year


   


Lookup Conference Papers

Close Browse Journals / Lookup Meetings

Article Tools

Share
Citations

Abstract

This Letter presents an enhanced temporal focusing-based multiphoton excitation (MPE) microscope in which the conventional diffraction grating is replaced by a digital micromirror device (DMD). Experimental results from imaging a thin fluorescence film show that the 4.0 μm axial resolution of the microscope is comparable with that of a setup incorporating a 600 lines / mm grating; hence, the optical sectioning ability of the proposed setup is demonstrated. Similar to a grating, the DMD diffracts illuminating light frequencies for temporal focusing; additionally, it generates arbitrary patterns. Since the DMD is placed on the image–conjugate plane of the objective lens’ focal plane, the MPE pattern can be projected on the focal plane precisely.

© 2014 Optical Society of America

OCIS Codes
(140.7090) Lasers and laser optics : Ultrafast lasers
(170.2520) Medical optics and biotechnology : Fluorescence microscopy
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(180.4315) Microscopy : Nonlinear microscopy

ToC Category:
Medical Optics and Biotechnology

History
Original Manuscript: January 16, 2014
Revised Manuscript: April 2, 2014
Manuscript Accepted: April 13, 2014
Published: May 19, 2014

Citation
Jenq-Nan Yih, Yvonne Yuling Hu, Yong Da Sie, Li-Chung Cheng, Chi-Hsiang Lien, and Shean-Jen Chen, "Temporal focusing-based multiphoton excitation microscopy via digital micromirror device," Opt. Lett. 39, 3134-3137 (2014)
http://www.opticsinfobase.org/ol/abstract.cfm?URI=ol-39-11-3134


Sort:  Author  |  Year  |  Journal  |  Reset  

References

  1. B. R. Masters and P. T. C. So, Handbook of Biomedical Nonlinear Optical Microscopy (Oxford, 2008).
  2. P. T. C. So, E. Y. S. Yew, and C. Rowlands, Biophys. J. 105, 2641 (2013). [CrossRef]
  3. D. Oron, E. Tal, and Y. Silberberg, Opt. Express 13, 1468 (2005). [CrossRef]
  4. G. Zhu, J. V. Howe, M. Durst, W. Zipfel, and C. Xu, Opt. Express 13, 2153 (2005). [CrossRef]
  5. A. Vaziri, J. Tang, H. Shroff, and C. V. Shank, Proc. Natl. Acad. Sci. U. S. A. 105, 20221 (2008). [CrossRef]
  6. E. Papagiakoumou, F. Anselmi, A. Bègue, V. de Sars, J. Glückstad, E. Y. Isacoff, and V. Emiliani, Nat. Methods 7, 848 (2010). [CrossRef]
  7. D. Kim and P. T. C. So, Opt. Lett. 35, 1602 (2010). [CrossRef]
  8. O. D. Therrien, B. Aubé, S. Pagès, P. De Koninck, and D. Côté, Biomed. Opt. Express 2, 696 (2011). [CrossRef]
  9. L.-C. Cheng, C.-Y. Chang, C.-Y. Lin, K.-C. Cho, W.-C. Yen, N.-S. Chang, C. Xu, C. Y. Dong, and S.-J. Chen, Opt. Express 20, 8939 (2012). [CrossRef]
  10. J.-Y. Yu, C.-H. Kuo, D. B. Holland, Y. Chen, M. Ouyang, G. A. Blake, R. Zadoyan, and C.-L. Guo, J. Biomed. Opt. 16, 116009 (2011). [CrossRef]
  11. H. Dana and S. Shoham, Opt. Lett. 37, 2913 (2012). [CrossRef]
  12. P. Zhu, O. Fajardo, J. Shum, Y.-P. Zhang Schärer, and R. W. Friedrich, Nat. Protoc. 7, 1410 (2012). [CrossRef]
  13. D. N. Vitek, D. E. Adams, A. Johnson, P. S. Tsai, S. Backus, C. G. Durfee, D. Kleinfeld, and J. A. Squier, Opt. Express 18, 18086 (2010). [CrossRef]
  14. Y.-C. Li, L.-C. Cheng, C.-Y. Chang, C.-H. Lien, P. J. Campagnola, and S.-J. Chen, Opt. Express 20, 19030 (2012). [CrossRef]
  15. T. Kreis, P. Aswendt, and R. Höfling, Opt. Eng. 40, 926 (2001). [CrossRef]
  16. D. Dudley, W. Duncan, and J. Slaughter, Proc. SPIE 4985, 14 (2003). [CrossRef]
  17. P. J. Rodrigo, I. R. Perch-Nielsen, and J. Glückstad, Opt. Express 14, 5588 (2006). [CrossRef]
  18. Y.-C. Li, L.-C. Cheng, C.-Y. Chang, C.-Y. Lin, N.-S. Chang, P. J. Campagnola, C. Y. Dong, and S.-J. Chen, J. Biomed. Opt. 18, 075004 (2013). [CrossRef]
  19. E. Y. S. Yew, C. J. R. Sheppard, and P. T. C. So, Opt. Express 21, 12951 (2013). [CrossRef]
  20. D. Lim, K. K. Chu, and J. Mertz, Opt. Lett. 33, 1819 (2008). [CrossRef]
  21. M. G. L. Gustafsson, J. Microsc. 198, 82 (2000). [CrossRef]

Cited By

Alert me when this paper is cited

OSA is able to provide readers links to articles that cite this paper by participating in CrossRef's Cited-By Linking service. CrossRef includes content from more than 3000 publishers and societies. In addition to listing OSA journal articles that cite this paper, citing articles from other participating publishers will also be listed.

Figures

Fig. 1. Fig. 2. Fig. 3.
 

« Previous Article  |  Next Article »

OSA is a member of CrossRef.

CrossCheck Deposited