OSA's Digital Library

Optics Letters

Optics Letters

| RAPID, SHORT PUBLICATIONS ON THE LATEST IN OPTICAL DISCOVERIES

  • Editor: Xi-Cheng Zhang
  • Vol. 39, Iss. 15 — Aug. 1, 2014
  • pp: 4290–4293

What structural length scales can be detected by the spectral variance of a microscope image?

Lusik Cherkezyan, Hariharan Subramanian, and Vadim Backman  »View Author Affiliations


Optics Letters, Vol. 39, Issue 15, pp. 4290-4293 (2014)
http://dx.doi.org/10.1364/OL.39.004290


View Full Text Article

Enhanced HTML    Acrobat PDF (521 KB)





Browse Journals / Lookup Meetings

Browse by Journal and Year


   


Lookup Conference Papers

Close Browse Journals / Lookup Meetings

Article Tools

Share
Citations

Abstract

A spectroscopic microscope, configured to detect interference spectra of backscattered light in the far zone, quantifies the statistics of refractive-index (RI) distribution via the spectral variance (Σ˜2) of the acquired bright-field image. Its sensitivity to subtle structural changes within weakly scattering, label-free media at subdiffraction scales shows great promise in fields from material science to medical diagnostics. We further investigate the length-scale sensitivity of Σ˜ and reveal that, in theory, it can detect RI fluctuations at any spatial frequency whatsoever. Based on a 5% noise floor, Σ˜ detects scales from 22 to 200–700 nm (exact values depend on sample structure and thickness). In an example involving mass-density distribution characteristic of biological cell nuclei, we suggest the level of chromatin organization, which can be quantified via Σ˜.

© 2014 Optical Society of America

OCIS Codes
(170.1530) Medical optics and biotechnology : Cell analysis
(180.0180) Microscopy : Microscopy
(290.0290) Scattering : Scattering
(300.0300) Spectroscopy : Spectroscopy

ToC Category:
Microscopy

History
Original Manuscript: March 10, 2014
Revised Manuscript: June 5, 2014
Manuscript Accepted: June 12, 2014
Published: July 16, 2014

Virtual Issues
Vol. 9, Iss. 10 Virtual Journal for Biomedical Optics

Citation
Lusik Cherkezyan, Hariharan Subramanian, and Vadim Backman, "What structural length scales can be detected by the spectral variance of a microscope image?," Opt. Lett. 39, 4290-4293 (2014)
http://www.opticsinfobase.org/ol/abstract.cfm?URI=ol-39-15-4290


Sort:  Author  |  Year  |  Journal  |  Reset  

References

  1. L. Cherkezyan, I. Capoglu, H. Subramanian, J. D. Rogers, D. Damania, A. Taflove, and V. Backman, Phys. Rev. Lett. 111, 033903 (2013). [CrossRef]
  2. H. G. Davies, M. H. F. Wilkins, J. Chayen, and L. F. La Cour, Q. J. Microsc. Sci. s3–95, 271 (1954).
  3. G. C. Crossmon, Stain Technol. 24, 241 (1949).
  4. A. J. Radosevich, J. Yi, J. D. Rogers, and V. Backman, Opt. Lett. 37, 5220 (2012). [CrossRef]
  5. L. Cherkezyan, Y. Stypula-Cyrus, H. Subramanian, C. White, M. Dela Cruz, R. K. Wali, M. J. Goldberg, L. K. Bianchi, H. K. Roy, and V. Backman, BMC Cancer 14, 189 (2014).
  6. H. Subramanian, P. Pradhan, Y. Liu, I. R. Capoglu, J. D. Rogers, H. K. Roy, R. E. Brand, and V. Backman, Opt. Lett. 34, 518 (2009). [CrossRef]

Cited By

Alert me when this paper is cited

OSA is able to provide readers links to articles that cite this paper by participating in CrossRef's Cited-By Linking service. CrossRef includes content from more than 3000 publishers and societies. In addition to listing OSA journal articles that cite this paper, citing articles from other participating publishers will also be listed.


« Previous Article  |  Next Article »

OSA is a member of CrossRef.

CrossCheck Deposited