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Optics Letters

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  • Editor: Xi-Cheng Zhang
  • Vol. 39, Iss. 15 — Aug. 1, 2014
  • pp: 4424–4426

Measuring blood velocity using correlative spectrally encoded flow cytometry

Tal Elhanan and Dvir Yelin  »View Author Affiliations


Optics Letters, Vol. 39, Issue 15, pp. 4424-4426 (2014)
http://dx.doi.org/10.1364/OL.39.004424


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Abstract

Spectrally encoded flow cytometry (SEFC) is a promising technique for imaging blood in the microcirculation. Yet, the dependency of one of the axes of the image on time prevents effective quantification of essential clinical parameters. Here, we address this challenge by splitting the optical path in an SEFC system into two parallel imaging lines, followed by straightforward data analysis for recovering the flow speed from the multiplexed data. The method is demonstrated by measuring the flow velocity of latex beads and blood cells in vitro. The system allows real-time velocity measurements of up to 11.7mm/s at high spatial resolution, and could be integrated into existing SEFC systems for effectively measuring blood parameters in small capillary vessels.

© 2014 Optical Society of America

OCIS Codes
(120.3890) Instrumentation, measurement, and metrology : Medical optics instrumentation
(170.1790) Medical optics and biotechnology : Confocal microscopy
(170.4580) Medical optics and biotechnology : Optical diagnostics for medicine
(280.2490) Remote sensing and sensors : Flow diagnostics

ToC Category:
Medical Optics and Biotechnology

History
Original Manuscript: May 6, 2014
Revised Manuscript: June 24, 2014
Manuscript Accepted: June 25, 2014
Published: July 23, 2014

Citation
Tal Elhanan and Dvir Yelin, "Measuring blood velocity using correlative spectrally encoded flow cytometry," Opt. Lett. 39, 4424-4426 (2014)
http://www.opticsinfobase.org/ol/abstract.cfm?URI=ol-39-15-4424


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