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Optics Letters

Optics Letters

| RAPID, SHORT PUBLICATIONS ON THE LATEST IN OPTICAL DISCOVERIES

  • Editor: Anthony J. Campillo
  • Vol. 32, Iss. 11 — Jun. 1, 2007
  • pp: 1417–1419

Optics InfoBase > Optics Letters > Volume 32 > Issue 11 > Dynamic speckle illumination microscopy with wavelet prefiltering

Dynamic speckle illumination microscopy with wavelet prefiltering

Cathie Ventalon, Rainer Heintzmann, and Jerome Mertz  »View Author Affiliations


Optics Letters, Vol. 32, Issue 11, pp. 1417-1419 (2007)
http://dx.doi.org/10.1364/OL.32.001417


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Abstract

Dynamic speckle illumination (DSI) provides a simple and robust technique to obtain fluorescence depth sectioning with a widefield microscope. We report a significant improvement to DSI microscopy based on a statistical image-processing algorithm that incorporates spatial wavelet prefiltering. The resultant gain in sectioning strength leads to a fundamentally improved scaling law for the out-of-focus background rejection.

© 2007 Optical Society of America

OCIS Codes
(180.1790) Microscopy : Confocal microscopy
(180.2520) Microscopy : Fluorescence microscopy
(180.6900) Microscopy : Three-dimensional microscopy

ToC Category:
Microscopy

History
Original Manuscript: January 18, 2007
Revised Manuscript: March 7, 2007
Manuscript Accepted: March 15, 2007
Published: April 27, 2007

Virtual Issues
Vol. 2, Iss. 7 Virtual Journal for Biomedical Optics

Citation
Cathie Ventalon, Rainer Heintzmann, and Jerome Mertz, "Dynamic speckle illumination microscopy with wavelet prefiltering," Opt. Lett. 32, 1417-1419 (2007)
http://www.opticsinfobase.org/ol/abstract.cfm?URI=ol-32-11-1417

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