OSA's Digital Library

Optics Letters

Optics Letters


  • Vol. 10, Iss. 2 — Feb. 1, 1985
  • pp: 53–55

Three-dimensional microscopy using a confocal laser scanning microscope

K. Carlsson, P. E. Danielsson, R. Lenz, A. Liljeborg, L. Majlöf, and N. Åslund  »View Author Affiliations

Optics Letters, Vol. 10, Issue 2, pp. 53-55 (1985)

View Full Text Article

Enhanced HTML    Acrobat PDF (422 KB)

Browse Journals / Lookup Meetings

Browse by Journal and Year


Lookup Conference Papers

Close Browse Journals / Lookup Meetings

Article Tools



In a scanning laser microscope detecting fluorescent light from the specimen, the depth-discriminating property of confocal scanning has been used to carry out optical slicing of a thick specimen. The recorded digital images constitute a three-dimensional raster covering a volume of the specimen. The specimen has been visualized in stereo and rotation by making look-through projections of the digital data in different directions. The contrast of the pictures has been enhanced by generating the gradient volume. This permits display of the border surfaces between regions instead of the regions themselves.

© 1985 Optical Society of America

Original Manuscript: September 17, 1984
Manuscript Accepted: November 15, 1984
Published: February 1, 1985

K. Carlsson, P. E. Danielsson, A. Liljeborg, L. Majlöf, R. Lenz, and N. Åslund, "Three-dimensional microscopy using a confocal laser scanning microscope," Opt. Lett. 10, 53-55 (1985)

Sort:  Author  |  Year  |  Journal  |  Reset  


  1. D. A. Agard, J. W. Sedat, Nature 302, 676 (1983). [CrossRef] [PubMed]
  2. M. Minsky, “Microscopy apparatus,” U.S. Patent3,013,467 (December191961).
  3. P. Davidovits, M. D. Egger, Appl. Opt. 10, 1615 (1971). [CrossRef] [PubMed]
  4. A. F. Slomba, D. E. Wasserman, G. I. Kaufman, J. F. Nester, J. Assoc. Adv. Med. Instrum. 6, 230 (1972). [PubMed]
  5. C. J. R. Sheppard, T. Wilson, Opt. Lett. 3, 115 (1978). [CrossRef] [PubMed]
  6. G. J. Brakenhoff, P. Blom, P. Barends, J. Microsc. (Oxford) 117, 219 (1979). [CrossRef]
  7. T. Wilson, J. Appl. Phys. 22, 119 (1980). [CrossRef]
  8. D. K. Hamilton, T. Wilson, C. J. R. Sheppard, Opt. Lett. 6, 625 (1981). [CrossRef] [PubMed]
  9. I. J. Cox, C. J. R. Sheppard, T. Wilson, Optik 60, 391 (1982).
  10. I. J. Cox, J. Microsc. 133, 149 (1984). [CrossRef] [PubMed]
  11. C. J. R. Sheppard, D. K. Hamilton, Appl. Opt. 22, 886 (1982). [CrossRef]
  12. N. Åslund, K. Carlsson, A. Liljeborg, L. Majlöf, in Proceedings of the Third Scandinavian Conference on Image Analysis (Studentlitteratur, Lund, Sweden, 1983), p. 338.
  13. W. W. Stewart, Cell 14, 714 (1978). [CrossRef]
  14. P. E. Danielsson, B. Kruse, B. Gudmundsson, in Proceedings of the Workshop on Picture Data Description and Managements (Institute of Electrical and Electronics Engineers, New York, 1980), p. 275.

Cited By

Alert me when this paper is cited

OSA is able to provide readers links to articles that cite this paper by participating in CrossRef's Cited-By Linking service. CrossRef includes content from more than 3000 publishers and societies. In addition to listing OSA journal articles that cite this paper, citing articles from other participating publishers will also be listed.


Fig. 1 Fig. 2 Fig. 3

Next Article »

OSA is a member of CrossRef.

CrossCheck Deposited