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Optics Letters

Optics Letters

| RAPID, SHORT PUBLICATIONS ON THE LATEST IN OPTICAL DISCOVERIES

  • Vol. 19, Iss. 3 — Feb. 1, 1994
  • pp: 222–224

Confocal microscopy with an increased detection aperture: type-B 4Pi confocal microscopy

Stefan W. Hell, Ernst H. K. Stelzer, Steffen Lindek, and Christoph Cremer  »View Author Affiliations


Optics Letters, Vol. 19, Issue 3, pp. 222-224 (1994)
http://dx.doi.org/10.1364/OL.19.000222


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Abstract

We show the point-spread function of a confocal microscope with an increased detection aperture. The increase in aperture is accomplished by coherent collection of the light from the specimen with two opposing objective lenses, i.e., type-B 4Pi confocal microscopy. We demonstrate experimentally its feasibility for detecting scattered or fluorescently emitted light. The 4Pi confocal point-spread functions are shown for constructive and destructive interference of the collected wave fronts and are compared with the point-spread functions of comparable confocal microscopes.

© 1994 Optical Society of America

History
Original Manuscript: August 9, 1993
Published: February 1, 1994

Citation
Stefan W. Hell, Steffen Lindek, Christoph Cremer, and Ernst H. K. Stelzer, "Confocal microscopy with an increased detection aperture: type-B 4Pi confocal microscopy," Opt. Lett. 19, 222-224 (1994)
http://www.opticsinfobase.org/ol/abstract.cfm?URI=ol-19-3-222

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