When a confocal fluorescence microscope with a high-numerical-aperture oil-immersion objective is focused deep into an aqueous medium, aberrations result that degrade image quality. We have designed and fabricated a simple two-level binary phase mask that partially corrects these aberrations, improving axial resolution. We present the design and some confirming results.
© 1995 Optical Society of America
Original Manuscript: November 9, 1994
Published: May 15, 1995
Christian K. Sieracki, Christopher G. Levey, and Eric W. Hansen, "Simple binary optical elements for aberration correction in confocal microscopy," Opt. Lett. 20, 1213-1215 (1995)