OSA's Digital Library

Optics Letters

Optics Letters


  • Vol. 20, Iss. 10 — May. 15, 1995
  • pp: 1213–1215

Simple binary optical elements for aberration correction in confocal microscopy

Christian K. Sieracki, Christopher G. Levey, and Eric W. Hansen  »View Author Affiliations

Optics Letters, Vol. 20, Issue 10, pp. 1213-1215 (1995)

View Full Text Article

Enhanced HTML    Acrobat PDF (255 KB)

Browse Journals / Lookup Meetings

Browse by Journal and Year


Lookup Conference Papers

Close Browse Journals / Lookup Meetings

Article Tools



When a confocal fluorescence microscope with a high-numerical-aperture oil-immersion objective is focused deep into an aqueous medium, aberrations result that degrade image quality. We have designed and fabricated a simple two-level binary phase mask that partially corrects these aberrations, improving axial resolution. We present the design and some confirming results.

© 1995 Optical Society of America

Original Manuscript: November 9, 1994
Published: May 15, 1995

Christian K. Sieracki, Christopher G. Levey, and Eric W. Hansen, "Simple binary optical elements for aberration correction in confocal microscopy," Opt. Lett. 20, 1213-1215 (1995)

Sort:  Author  |  Year  |  Journal  |  Reset  


  1. S. F. Gibson, F. Lanni, J. Opt. Soc. Am. A 8, 1601 (1991). [CrossRef]
  2. S. F. F. Gibson, “Modeling the three-dimensional imaging properties of the fluorescence light microscope,” Ph.D. dissertation (Carnegie-Mellon University, Pittsburg, Pa., 1990).
  3. H. T. M. van der Voort, G. J. Brakenhoff, J. Microsc. 158, 43 (1990). [CrossRef]
  4. K. Carlsson, J. Microsc. 163, 167 (1991). [CrossRef]
  5. C. J. R. Sheppard, M. Gu, Appl. Opt. 30, 3563 (1991). [CrossRef] [PubMed]
  6. C. J. R. Sheppard, M. Gu, Opt. Commun. 88, 180 (1992). [CrossRef]
  7. S. Hell, G. Reiner, C. Cremer, E. H. K. Stelzer, J. Microsc. 169, 391 (1993). [CrossRef]
  8. M. Brenner, Am. Lab. 26, 14 (1994).
  9. E. W. Hansen, J. P. Zelten, B. A. Wiseman, Proc. Soc. Photo-Opt. Instrum. Eng. 909, 304 (1988).

Cited By

Alert me when this paper is cited

OSA is able to provide readers links to articles that cite this paper by participating in CrossRef's Cited-By Linking service. CrossRef includes content from more than 3000 publishers and societies. In addition to listing OSA journal articles that cite this paper, citing articles from other participating publishers will also be listed.


Fig. 1 Fig. 2 Fig. 3
Fig. 4 Fig. 5

« Previous Article  |  Next Article »

OSA is a member of CrossRef.

CrossCheck Deposited