OSA's Digital Library

Optics Letters

Optics Letters

| RAPID, SHORT PUBLICATIONS ON THE LATEST IN OPTICAL DISCOVERIES

  • Vol. 20, Iss. 21 — Nov. 1, 1995
  • pp: 2246–2248

Two-photon fluorescence excitation in continuous-wave infrared optical tweezers

Y. Liu, G. J. Sonek, M. W. Berns, K. Konig, and B. J. Tromberg  »View Author Affiliations


Optics Letters, Vol. 20, Issue 21, pp. 2246-2248 (1995)
http://dx.doi.org/10.1364/OL.20.002246


View Full Text Article

Enhanced HTML    Acrobat PDF (253 KB)





Browse Journals / Lookup Meetings

Browse by Journal and Year


   


Lookup Conference Papers

Close Browse Journals / Lookup Meetings

Article Tools

Share
Citations

Abstract

We report the observation of two-photon fluorescence excitation in a continuous-wave (cw) single-beam gradient force optical trap and demonstrate its use as an in situ probe to study the physiological state of an optically confined sample. In particular, a cw Nd:YAG (1064-nm) laser is used simultaneously to confine, and excite visible fluorescence from submicrometer regions of, cell specimens. Two-photon fluorescence emission spectra are presented for motile human sperm cells and immotile Chinese hamster ovary cells that have been labeled with nucleic acid (Propidium Iodide) and pH-sensitive (Snarf) fluorescent probes. The resulting spectra are correlated to light-induced changes in the physiological state experienced by the trapped cells. This spectral technique should prove extremely useful for monitoring cellular activity and the effects of confinement by optical tweezers.

© 1995 Optical Society of America

History
Original Manuscript: June 28, 1995
Published: November 1, 1995

Citation
Y. Liu, M. W. Berns, K. Konig, G. J. Sonek, and B. J. Tromberg, "Two-photon fluorescence excitation in continuous-wave infrared optical tweezers," Opt. Lett. 20, 2246-2248 (1995)
http://www.opticsinfobase.org/ol/abstract.cfm?URI=ol-20-21-2246

You do not have subscription access to this journal. Citation lists with outbound citation links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Log in to access OSA Member Subscription

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Log in to access OSA Member Subscription

You do not have subscription access to this journal. Figure files are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Log in to access OSA Member Subscription

You do not have subscription access to this journal. Article level metrics are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Log in to access OSA Member Subscription

« Previous Article  |  Next Article »

OSA is a member of CrossRef.

CrossCheck Deposited