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Optics Letters

Optics Letters


  • Vol. 20, Iss. 21 — Nov. 1, 1995
  • pp: 2246–2248

Two-photon fluorescence excitation in continuous-wave infrared optical tweezers

Y. Liu, G. J. Sonek, M. W. Berns, K. Konig, and B. J. Tromberg  »View Author Affiliations

Optics Letters, Vol. 20, Issue 21, pp. 2246-2248 (1995)

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We report the observation of two-photon fluorescence excitation in a continuous-wave (cw) single-beam gradient force optical trap and demonstrate its use as an in situ probe to study the physiological state of an optically confined sample. In particular, a cw Nd:YAG (1064-nm) laser is used simultaneously to confine, and excite visible fluorescence from submicrometer regions of, cell specimens. Two-photon fluorescence emission spectra are presented for motile human sperm cells and immotile Chinese hamster ovary cells that have been labeled with nucleic acid (Propidium Iodide) and pH-sensitive (Snarf) fluorescent probes. The resulting spectra are correlated to light-induced changes in the physiological state experienced by the trapped cells. This spectral technique should prove extremely useful for monitoring cellular activity and the effects of confinement by optical tweezers.

© 1995 Optical Society of America

Original Manuscript: June 28, 1995
Published: November 1, 1995

Y. Liu, M. W. Berns, K. Konig, G. J. Sonek, and B. J. Tromberg, "Two-photon fluorescence excitation in continuous-wave infrared optical tweezers," Opt. Lett. 20, 2246-2248 (1995)

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