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Optics Letters

Optics Letters


  • Vol. 20, Iss. 24 — Dec. 15, 1995
  • pp: 2532–2534

Single-molecule detection by two-photon-excited fluorescence

J. Mertz, C. Xu, and W. W. Webb  »View Author Affiliations

Optics Letters, Vol. 20, Issue 24, pp. 2532-2534 (1995)

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We present a technique for observing single fluorophore molecules in solution. A mode-locked laser beam is focused into the solution, thereby defining a two-photon excitation volume localized in three dimensions. Molecules diffusing into and out of this volume produce fluorescence bursts, which are detected with a high signal-to-background ratio. The theoretical foundations for the technique are laid out, including the attendant fluorescence rate distribution, and agree with experimental results obtained for Rhodamine B molecules in water.

© 1995 Optical Society of America

Original Manuscript: August 4, 1995
Published: December 15, 1995

J. Mertz, C. Xu, and W. W. Webb, "Single-molecule detection by two-photon-excited fluorescence," Opt. Lett. 20, 2532-2534 (1995)

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