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Optics Letters

Optics Letters


  • Vol. 21, Iss. 13 — Jul. 1, 1996
  • pp: 988–990

Resolution in three-photon fluorescence scanning microscopy

Min Gu  »View Author Affiliations

Optics Letters, Vol. 21, Issue 13, pp. 988-990 (1996)

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Resolution in potential three-photon fluorescence scanning microscopy is discussed in terms of the three-dimensional optical transfer function. Images of layers and sharp edges are presented for a comparison of the resolution with that in two-photon fluorescence microscopic imaging. For the same fluorescence wavelength the resolution is almost the same in both cases. However, for a given illumination wavelength the resolution for imaging a thick object in the case of three-photon fluorescence imaging can be improved by as much as 40–50% relative to that in two-photon imaging.

© 1996 Optical Society of America

Original Manuscript: January 16, 1996
Published: July 1, 1996

Min Gu, "Resolution in three-photon fluorescence scanning microscopy," Opt. Lett. 21, 988-990 (1996)

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