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Optics Letters

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  • Vol. 21, Iss. 16 — Aug. 15, 1996
  • pp: 1307–1309

Conditional-firing aerosol-fluorescence spectrum analyzer for individual airborne particles with pulsed 266-nm laser excitation

Gang Chen, Paul Nachman, Ronald G. Pinnick, Steven C. Hill, and Richard K. Chang  »View Author Affiliations


Optics Letters, Vol. 21, Issue 16, pp. 1307-1309 (1996)
http://dx.doi.org/10.1364/OL.21.001307


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Abstract

We report the operation of an aerosol-fluorescence spectrum analyzer capable of selectively measuring the fluorescence spectra of single micrometer-sized aerosol particles as they flow through the instrument. As the particle first traverses a cw 488-nm probe laser beam, the total fluorescence and elastic scattering are measured with photomultipliers. When the photomultiplier output levels meet preset logic conditions, a UV laser (at 266 nm) is fired and the particle fluorescence spectrum is recorded. Fluorescence spectra of biological airborne particles are presented. The ability of the analyzer to capture the fluorescence spectrum of one type of particle while ignoring others, based on the particle characteristics, is also demonstrated.

© 1996 Optical Society of America

History
Original Manuscript: February 21, 1996
Published: August 15, 1996

Citation
Gang Chen, Paul Nachman, Richard K. Chang, Ronald G. Pinnick, and Steven C. Hill, "Conditional-firing aerosol-fluorescence spectrum analyzer for individual airborne particles with pulsed 266-nm laser excitation," Opt. Lett. 21, 1307-1309 (1996)
http://www.opticsinfobase.org/ol/abstract.cfm?URI=ol-21-16-1307


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References

  1. J. R. Lakowicz, Principles of Fluorescence Spectroscopy (Plenum, New York, 1983), Chap. 3, p. 341. [CrossRef]
  2. R. G. Pinnick, S. C. Hill, P. Nachman, J. D. Pendleton, G. L. Fernandez, M. W. Mayo, J. G. Bruno, Aerosol Sci. Technol. 23, 653 (1995). [CrossRef]
  3. S. C. Hill, R. G. Pinnick, P. Nachman, G. Chen, R. K. Chang, M. W. Mayo, G. L. Fernandez, Appl. Opt. 34, 7149 (1995). [CrossRef] [PubMed]
  4. P. Nachman, G. Chen, R. G. Pinnick, S. C. Hill, R. K. Chang, M. W. Mayo, G. L. Fernandez, Appl. Opt. 35, 1069 (1996). [CrossRef] [PubMed]
  5. M. W. Mayo, S. C. Hill, R. K. Chang, “Fluorescence of bacteria, pollens and naturally occurring airborne particles: excitation/emission spectra,” Appl. Spectrosc. (to be published).
  6. P. G. Carson, K. R. Neubauer, M. V. Johnston, A. S. Wexler, J. Aerosol Sci. 26, 535 (1995). [CrossRef]
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  8. J. W. Longworth, in Excited States of Proteins and Nucleic Acids, R. F. Steiner, I. Weinryb, eds. (Plenum, New York, 1971), Chap. 6, p. 319. [CrossRef]

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