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Optics Letters

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  • Vol. 21, Iss. 17 — Sep. 1, 1996
  • pp: 1411–1413

Two-photon-excitation scanning microscopy of living neurons with a saturable Bragg reflector mode-locked diode-pumped Cr:LiSrAlFl laser

K. Svoboda, W. Denk, W. H. Knox, and S. Tsuda  »View Author Affiliations


Optics Letters, Vol. 21, Issue 17, pp. 1411-1413 (1996)
http://dx.doi.org/10.1364/OL.21.001411


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Abstract

A Cr:LiSrAlFl laser, pumped with a diffraction-limited laser diode and mode locked with a saturable Bragg reflector, produces 90-fs pulses at 860 nm with a cw power as high as 88 mW in two beams. It is shown that this recently developed, compact, solid-state laser can be used as an excitation source for two-photon laser scanning microscopy. Morphological and functional images of neocortical and cerebellar neurons were obtained with submicrometer three-dimensional resolution. Single dendritic spines could easily be resolved deep in scattering tissue.

© 1996 Optical Society of America

History
Original Manuscript: April 1, 1996
Published: September 1, 1996

Citation
K. Svoboda, W. H. Knox, S. Tsuda, and W. Denk, "Two-photon-excitation scanning microscopy of living neurons with a saturable Bragg reflector mode-locked diode-pumped Cr:LiSrAlFl laser," Opt. Lett. 21, 1411-1413 (1996)
http://www.opticsinfobase.org/ol/abstract.cfm?URI=ol-21-17-1411


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References

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