OSA's Digital Library

Optics Letters

Optics Letters

| RAPID, SHORT PUBLICATIONS ON THE LATEST IN OPTICAL DISCOVERIES

  • Vol. 22, Iss. 2 — Jan. 15, 1997
  • pp: 135–136

Cellular response to near-infrared femtosecond laser pulses in two-photon microscopes

K. König, P. T. C. So, W. W. Mantulin, and E. Gratton  »View Author Affiliations


Optics Letters, Vol. 22, Issue 2, pp. 135-136 (1997)
http://dx.doi.org/10.1364/OL.22.000135


View Full Text Article

Acrobat PDF (205 KB)





Browse Journals / Lookup Meetings

Browse by Journal and Year


   


Lookup Conference Papers

Close Browse Journals / Lookup Meetings

Article Tools

Share
Citations

Abstract

The influence of femtosecond near-infrared (NIR) microirradiation on cell vitality and cellular reproduction has been studied. Chinese hamster ovary cells exposed to a highly focused 150-fs scanning beam at 730, 760, and 800 nm (80 MHz, 80-μs pixel dwell time) of ≤1 mW remained unaffected by the femtosecond microbeam. However, increased mean power led to impaired cell division. At ≥6 -mW mean power, cells were unable to form clones. They died or became giant cells. Complete cell destruction, including cell fragmentation, occurred at mean powers >10 mW. Cell death was accompanied by intense luminescence in the mitochondrial region. When we consider the diffraction-limited spot size in the submicrometer region, intensities and photon flux densities of 0.8-kW pulses (10-mW mean power) are of the order of terawatts per square centimeter (10<sup>12</sup>W/cm <sup>2</sup>) and 10<sup>32</sup> photons cm<sup>-2</sup> s<sup>-1</sup> , respectively. Extremely high fields may induce destructive intracellular plasma formation. The power limitations should be considered during NIR femtosecond microscopy of vital cells and in the design of compact NIR femtosecond solid-state lasers for two-photon microscopes.

© 1997 Optical Society of America

Citation
K. König, P. T. C. So, W. W. Mantulin, and E. Gratton, "Cellular response to near-infrared femtosecond laser pulses in two-photon microscopes," Opt. Lett. 22, 135-136 (1997)
http://www.opticsinfobase.org/ol/abstract.cfm?URI=ol-22-2-135


Sort:  Author  |  Year  |  Journal  |  Reset

References

  1. W. Denk, J. H. Strickler, and W. W. Webb, Science 248, 73 (1990).
  2. W. Denk, D. W. Piston, and W. W. Webb, in Handbook of Biological Confocal Microscopy, 2nd ed., J. B. Pawley, ed. (Plenum, New York, 1995), pp. 445–458.
  3. K. König, H. Liang, M. W. Berns, and B. J. Tromberg, Nature (London) 377, 20 (1995).
  4. K. König, H. Liang, M. W. Berns, and B. J. Tromberg, Opt. Lett. 21, 1090 (1996).
  5. P. T. C. So, T. French, W. W. Yu, K. M. Berland, C. Y. Dong, and E. Gratton, Bioimaging 3, 49 (1995).
  6. D. von der Linde and H. Schüler, J. Opt. Soc. Am. B 13, 216 (1996).

Cited By

Alert me when this paper is cited

OSA is able to provide readers links to articles that cite this paper by participating in CrossRef's Cited-By Linking service. CrossRef includes content from more than 3000 publishers and societies. In addition to listing OSA journal articles that cite this paper, citing articles from other participating publishers will also be listed.


« Previous Article

OSA is a member of CrossRef.

CrossCheck Deposited