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Optics Letters

Optics Letters


  • Vol. 22, Iss. 24 — Dec. 15, 1997
  • pp: 1905–1907

Method of obtaining optical sectioning by using structured light in a conventional microscope

M. A. A. Neil, R. Juskaitis, and T. Wilson  »View Author Affiliations

Optics Letters, Vol. 22, Issue 24, pp. 1905-1907 (1997)

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We describe a simple method of obtaining optical sectioning in a conventional wide-field microscope by projecting a single-spatial-frequency grid pattern onto the object. Images taken at three spatial positions of the grid are processed in real time to produce optically sectioned images that are substantially similar to those obtained with confocal microscopes.

© 1997 of America

OCIS Codes
(110.0180) Imaging systems : Microscopy
(180.0180) Microscopy : Microscopy
(180.1790) Microscopy : Confocal microscopy

M. A. A. Neil, R. Juskaitis, and T. Wilson, "Method of obtaining optical sectioning by using structured light in a conventional microscope," Opt. Lett. 22, 1905-1907 (1997)

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  1. T. Wilson and C. J.R. Sheppard, in Theory and Practice of Scanning Optical Microscopy, Academic, London, 1984
  2. , ed., in Confocal Microscopy, Academic, London, 1990
  3. B. Neumann, in Proc. SPIE, 1989
  4. C. G. Morgan, January 10, 1995, U.S. patent 5,381,236
  5. A. B. Carlson, in Communication Systems, McGraw-Hill, New York, 1988
  6. P. A. Stokseth, J. Opt. Soc. Am., 59, 1314, 1969
  7. T. Wilson and R. Juskaitis, Bioimaging, 3, 35, 1995

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