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Optics Letters

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  • Vol. 23, Iss. 10 — May. 15, 1998
  • pp: 810–812

Fluorescence lifetime imaging with picosecond resolution for biomedical applications

K. Dowling, M. J. Dayel, M. J. Lever, P. M. W. French, J. D. Hares, and A. K. L. Dymoke-Bradshaw  »View Author Affiliations


Optics Letters, Vol. 23, Issue 10, pp. 810-812 (1998)
http://dx.doi.org/10.1364/OL.23.000810


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Abstract

We describe a novel whole-field fluorescence lifetime imaging system, based on a time-gated image intensifier and a solid-state laser oscillator–amplifier, that images lifetime differences of less than 10 ps. This system was successfully applied to discrimination between biological tissue constituents.

© 1998 Optical Society of America

OCIS Codes
(140.7090) Lasers and laser optics : Ultrafast lasers
(170.1470) Medical optics and biotechnology : Blood or tissue constituent monitoring
(170.2520) Medical optics and biotechnology : Fluorescence microscopy
(170.3650) Medical optics and biotechnology : Lifetime-based sensing
(170.3880) Medical optics and biotechnology : Medical and biological imaging

Citation
K. Dowling, M. J. Dayel, M. J. Lever, P. M. W. French, J. D. Hares, and A. K. L. Dymoke-Bradshaw, "Fluorescence lifetime imaging with picosecond resolution for biomedical applications," Opt. Lett. 23, 810-812 (1998)
http://www.opticsinfobase.org/ol/abstract.cfm?URI=ol-23-10-810


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References

  1. J. R. Lakowicz, ed., Techniques, Vol. 1 of Topics in Fluorescence Spectroscopy (Plenum, New York, 1991).
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  7. The shorter fluorescence lifetime that we reported in Ref. 5 for Coumarin 314 was measured with a tightly focused pump beam that resulted in significant stimulated emission. For this measurement we reduced the pump intensity and eliminated this artifact. We are unaware of any published value for this lifetime.
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